Unit 5 Practice Test Chs. 8-9

The cancer gene ras produces mRNA containing an extra exon that includes a number of UAA codons. Cancer cells produce ras mRNA missing this exon. This mistake most likely is due to a mistake by

snRNPs

An enzyme that makes covalent bonds between nucleotide sequences in DNA is

DNA ligase

Which of the following statements is false?

DNA replication proceeds in one direction around the bacterial chromosome.

Multiple replication forks are possible on a bacterial chromosome.

The leading strand of DNA is made continuously.

DNA polymerase joins nucleotides in one direction only.

The lagging strand of DNA is started by an RNA primer.

DNA replication proceeds in one direction around the bacterial chromosome.

In Figure 8.2, if base 4 is thymine, what is base 4'?

Adenine

Table 8.2

Codon on mRNA and corresponding amino acid

UUA	leucine	UAA	nonsense
GCA	alanine	AAU	asparagine
AAG	lysine	UGC	cysteine
GUU	valine	UCG, UCU	serine

(Use Table 8.2.) If the sequence of amino acids encoded by a strand of DNA is serine-alanine-lysine-leucine, the coding for the sense strand of DNA is

5' TCTGCAAAGTTA

Transformation is the transfer of DNA from a donor to a recipient cell

As naked DNA in solution.

Which of the following is an advantage of using E. colito make a human gene product?

Its genes are well known.

Table 8.2

Codon on mRNA and corresponding amino acid

UUA	leucine	UAA	nonsense
GCA	alanine	AAU	asparagine
AAG	lysine	UGC	cysteine
GUU	valine	UCG, UCU	serine

(Use Table 8.2.) If the sequence of amino acids encoded by a strand of DNA is serine-alanine-lysine-leucine, what is the order of bases in the antisense strand of DNA?

3' AGACGTTTCAAT

Vectors often contain a gene conferring drug resistance to their cloning host, in order to detect cells harboring the plasmid. T/F

True

In Figure 9.4, the purpose of this experiment is to

Put an insecticide on plant leaves.

Protein synthesis in eukaryotes is similar to the process in prokaryotes in that both eukaryotes and prokaryotes

Use codons to arrange amino acids.

An enzyme that copies DNA to make a molecule of RNA is

RNA polymerase.

If you start with 3 double stranded DNA fragments, after 4 cycles of PCR you will have ____ fragments.

48

E. coli makes insulin because

The insulin gene was inserted into it.

In Figure 8.2, if base 4 is thymine, what is base 11'?

Thymine

Genetic engineering methods have been used to produce vaccines. T/F

True

Which of the following contain all the requirements for PCR?

Primers, Taq DNA polymerase, dNTPs

In Figure 9.1, after digestion with the appropriate restriction enzyme, what is the smallest piece containing the ampicillin-resistance (amp) gene?

1.50 kbp

Which of the following is NOT a product of transcription?

A new strand of DNA

mRNA

rRNA

tRNA

A new strand of DNA

Which of the following pairs is mismatched?

DNA polymerase - makes a molecule of DNA from a DNA template

DNA gyrase - coils and twists DNA

RNA polymerase - makes a molecule of RNA from an RNA template

Transposase - insertion of DNA segments into DNA

DNA ligase - joins segments of DNA

RNA polymerase - makes a molecule of RNA from an RNA template

Table 8.2

Codon on mRNA and corresponding amino acid

UUA	leucine	UAA	nonsense
GCA	alanine	AAU	asparagine
AAG	lysine	UGC	cysteine
GUU	valine	UCG, UCU	serine

(Use Table 8.2.) The anticodon for valine is

CAA

Conjugation differs from reproduction because conjugation

Transfers DNA horizontally, to cells in the same generation.

According to the operon model, for the synthesis of an inducible enzyme to occur, the

Substrate must bind to the repressor.

Thermococcus litoralis and Thermus aquaticus are thermophilic bacteria that are

Sources of heat stable DNA polymerases

The mechanism by which the presence of glucose inhibits the arabinose operon is

Catabolite repression

Genetic change in bacteria can be brought about by

Conjugation

Transformation

Transduction

Mutation

All of the above

All of the above

The mechanism by which the presence of arabinose controls the arabinose operon is

Induction

Which of the following is NOT an agricultural product made by DNA techniques?

Pectinase

In transcription,

DNA is copied to RNA

DNA is constructed of

Two strands of nucleotides running antiparallel

The Western Blot technique detects

Proteins

In Figure 8.4, the antibiotic chloramphenicol binds the 50S ribosome as shown. From this information you can conclude that chloramphenicol

Prevents translation in prokaryotes.

EcoRI and HindIII are

Restriction endonucleases

If you have inserted a gene in the Ti plasmid, the next step in genetic engineering is

Inserting the Ti plasmid into Agrobacterium

Biochemical tests are used to determine

Enzymatic activities.

Which enzyme does NOT make sticky ends?

Enzyme Recognition

BamHI G↓GATCC

CCTAG↑G

Enzyme Recognition

HaeIII GG↓CC

CC↑GG

Enzyme Recognition
EcoRI G↓AATTC
CTTAA↑G

Enzyme Recognition

PstI CTGC↓G

G↑ACGTC

Enzyme Recognition

HindIII A↓AGCTT

TTCGA↑A

Enzyme Recognition
HaeIII GG↓CC
CC↑GG