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The adenylate kinase reaction
2 ADP ⇌ ATP + AMP
Why AMP is a more sensitive energy signal than ATP
[ATP] ≫ [AMP] (~100×). A small % fall in ATP produces a large proportional rise in AMP — amplification via adenylate kinase
Energy charge formula
EC = ([ATP] + ½[ADP]) / ([ATP] + [ADP] + [AMP]); ranges 0–1, cells hold ~0.8–0.95
Effect of high energy charge on catabolic vs anabolic pathways
Inhibits catabolic (ATP-making), stimulates anabolic (ATP-using). Curves cross steeply near 0.85, buffering EC
AMPK's role in one line
Master fuel sensor: high AMP/ATP turns ON catabolism (glucose uptake, β-oxidation) and OFF anabolism (fat/cholesterol synthesis)
One-line summary of ATP vs AMP signalling
ATP says stop, AMP says go — and AMP shouts louder because it starts from almost nothing