CC2 Midterm Practicals

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Answer the ff:

1. Method/Author

2. Category of Estimation

3. Mode of Measurement

4. Substrate

5. End Color/Product

6. Wavelength

7. Reaction time

8. Lower-Upper limit

1. Oliver modified by Rosalki (Optimized UV test)

2. UV Spectrophotometry (Reverse Reaction)

3. Following the rate of increase in absorbance due to the reduction of NADP to NADPH at 340 nm

4. Creatine Phosphate

5. NADPH, Colorless

6. 340 nm 

7. 10 minutes

8. 2-2000 U/L

If the ∆A is 0.0012, compute for the CK − MB Activity in U/L and μkat/L.

Answer the ff:

1. Method/Author

2. Category of Estimation

3. Mode of Measurement

4. Substrate

5. End Color/Product

6. Wavelength

7. Reaction time

8. Lower-Upper limit

1. Amador et. al, Wacker, Ulmer, Valle (Optimized UV test)

2. UV Spectrophotometry (Forward Reaction)

3. Following the rate of increase in absorbance due to the reduction of NAD to NADH at 365 nm

4. L-Lactate

5. NADH, Colorless

6. 365 nm

7. 4 mins

8. 5 - 1200 U/L

If the ∆A is 0. 0136, compute for the LDH Activity in U/L and μkat/L.

Answer the ff:

1. Method/Author

2. Category of Estimation

3. Mode of Measurement

4. Substrate

5. End Color/Product

6. Wavelength

7. Reaction time

8. Lower-Upper limit

1. UV Method by Karmen Modified by Henry

2. UV Kinetic Spectrophotometry

3. Rate of decrease in absorbance due to the oxidation of NADH/unit to NAD at 365 nm

4. Oxaloacetate

5. NAD, Colorless

6. 365 nm

7. 4 mins

8. 2 - 700 U/L

If the ∆A is 0. 0012, compute for the AST Activity in U/L and μkat/L.

Answer the ff:

1. Method/Author

2. Category of Estimation

3. Mode of Measurement

4. Substrate

5. End Color/Product

6. Wavelength

7. Reaction time

8. Lower-Upper limit

1. UV Method by Karmen Modified by Henry

2. UV Kinetic Spectrophotometry

3. Rate of decrease in absorbance due to the oxidation of NADH/unit to NAD at 365 nm

4. Pyruvate

5. NAD, Colorless

6. 365 nm

7. 4 mins

8. 4 - 600 U/L

If the ∆A is 0. 005, compute for the ALT Activity in U/L and μkat/L.

Answer the ff:

1. Method/Author

2. Category of Estimation

3. Mode of Measurement

4. Substrate

5. End Color/Product

6. Wavelength

7. Reaction time

8. Lower-Upper limit

1. Caraway

2. Endpoint Spectrophotometry

3. The decrease in color intensity at 660 nm is directly proportional to the amount of amylase

4. Lintner’s Soluble Starch 

5. Starch-Iodine complex, Colorless

6. 660 nm

7. 7.5 mins

8. Up to 300 units

Solve for the a-Amylase activity in U/100 if A control is 0.34 and A test is 0.29.

Answer the ff:

1. Method/Author

2. Category of Estimation

3. Mode of Measurement

4. Substrate

5. End Color/Product

6. Wavelength

7. Reaction time

8. Lower-Upper limit

1. Wallenfels et al

2. Enzymatic, Colorimetric, Spectrophotometry

3. Rate of increase in absorbance due to p-nitrophenol at 405 nm 

4. 4,6-ethylidene-(G7)-p-nitrophenyl-(G1)- α-D-maltoheptoside   (EPS-G7)

5. para-nitrophenol, Yellow

6. 405 nm

7. 5 mins

8. 3 - 2000 U/L

If the ∆A is 0. 005, compute for the AMS Activity in U/L and μkat/L.

Answer the ff:

1. Method/Author

2. Category of Estimation

3. Mode of Measurement

4. Substrate

5. End Color/Product

6. Wavelength

7. Reaction time

8. Lower-Upper limit

1. Tietz-Fiereck 

2. Titrimetric endpoint analysis

3. Rate of formation of a definite blue color titrated using 0.05 N NaOH is directly proportional to Lipase Activity

4. Olive Oil

5. Oleic acid, Definite Blue color

6. -

7. 3 hrs and 10 mins

8. 0.05 - 1.0 Tietz-Fiereck units/ml 14 - 200 IU/mL

Solve for the LPS UNIT if the Test is 1.23 and the blank is 1.13

Answer the ff:

1. Method/Author

2. Category of Estimation

3. Mode of Measurement

4. Substrate

5. End Color/Product

6. Wavelength

7. Reaction time

8. Lower-Upper limit

1. DC FS (Direct Color Fluid Stable)

2. Enzymatic, Colorimetric

3. Rate of increase in absorbance of methylresorufin at 580 nm 

4. 6-methylresorufin ester or DRGG (1,2-odilauryl-rac-glycero-3-glutaric acid [6’-methylresorufin]ester)

5. Methylresorufin, Red color

6. 580 nm

7. 4 mins

8. 3 - 300 U/L

Answer the ff:

1. Method/Author

2. Category of Estimation

3. Mode of Measurement

4. Substrate

5. End Color/Product

6. Wavelength

7. Reaction time

8. Lower-Upper limit

1. Bessey, Lowry, & Brock modified by Bowers and Mccomb 

2. Kinetic, Photometric, Colorimetric

3. Rate of increasing absorbance of p-nitrophenol at 405 nm

4. para-nitrophenyl phosphate

5. para-nitrophenol, Yellow

6. 405 nm

7. 4 mins

8. 2 - 1400 U/L

Answer the ff:

1. Method/Author

2. Category of Estimation

3. Mode of Measurement

4. Substrate

5. End Color/Product

6. Wavelength

7. Reaction time

8. Lower-Upper limit

1. Babson, Phillips,and Read modified by Hillman

2. Colorimetric, 3-point Kinetic, Spectrophotometric

3. Rate of increasing absorbance of azoic chromogen (salmon pink) at 405 nm

4. alpha-naphthyl, phosphate

5. Azoic chromogen, Salmon pink

6. 405 nm

7. 8 mins

8. 0.8 - 150 U/L

Solve for the Prostatic ACP in UL and nkat/L, if the values for ∆A TOTAL ACP is 0.003 and ∆A NONPROSTATIC ACP is 0.002.