The light-independent reaction

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15 Terms

1
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What is the light-independent reaction also known as?

The Calvin cycle

2
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Who discovered the Calvin cycle?

Melvin Calvin

3
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Where in the chloroplast does the light-independent reaction take place?

The stroma

4
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Which two molecules produced during the light-dependent reaction are needed for the light-independent reaction?

NADPH and ATP

5
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What is the first stage of the Calvin cycle?

Ribulose bisphosphate (RuBP) reacts with CO2 to produce 2 molecules of glycerate 3-phosphate (GP). This reaction is catalysed by an enzyme called rubisco.

6
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What is the second stage of the Calvin cycle, after glycerate 3-phosphate has been produced?

2 molecules of glycerate 3-phosphate are reduced by NADPH to produce 2 molecules of triose phosphate. NADPH —> NADP (donates hydrogen to GP). ATP —> ADP + Pi (reaction requires energy)

7
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What is the third stage of the Calvin cycle, after triose phosphate has been produced?

Roughly 80% of triose phosphate produced is converted back to RuBP. ATP —> ADP + Pi (reaction requires energy). The other 20% is used to make organic molecules like glucose, glycerol or amino acids.

8
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How many carbons and phosphates are there in ribulose bisphosphate?

5 carbons and 2 phosphate groups

9
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How many carbons and phosphates are there in glycerate 3-phosphate?

3 carbons and 1 phosphate group

10
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How many carbons and phosphates are there in triose phosphate?

3 carbons and 1 phosphate group

11
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What happens to the NADP, ADP and Pi produced during the light-independent reaction?

They return to the thylakoid membrane, where they are converted back to ATP and NADPH in the light-dependent reaction

12
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Why does rate of photosynthesis increase with light intensity?

As light intensity increases, the rate of photoionisation and photolysis increases

13
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Why does rate of photosynthesis increase with carbon dioxide concentration?

As carbon dioxide concentration increases, ribulose bisphosphate is converted to glycerate 3-phosphate at a higher rate

14
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Why does rate of photosynthesis initially increase with temperature?

As temperature increases, rubisco activity in the light-independent reaction increases

15
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Why does rate of photosynthesis decrease if temperature gets too high?

  • Rubisco can denature if temperature gets too high

  • Could stop proteins in the thylakoid membrane from functioning properly, decreasing the rate of the light-dependent reaction