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Robert Hooke
built a microscope in 1663 and looked at dead bark from oak trees
pond water
Anton van Leeuwenhoek built a microscope to observe this and other living things
life
cells are the basic unit of
cell theory
all living things are composed of cells
obtaining energy, removing waste, reproducing
cells are the basic unit of living things and carry on similar functions, including
other cells
all cells are produced from
microscopes
the cell theory would not have been possible without
compound light microscope
magnifies images using two lenses (for objects as small as a millionth of a meter)
electron microscope
uses a beam of electrons focused by magnetic fields (used for objects as small as 1 billionth of a meter)
magnification
makes things look larger
resolution
makes objects clearer and detailed
contrast
the darkness or lightness of areas of an image
eyepiece (ocular lens) and objectives
compound light microscopes magnify light using two lenses:
1000x
light microscopes can only magnify objects to about _____ due to diffraction and scattering
transparent
cells are nearly __________, which is why scientists use stains, dyes and fluorescence to view cell components
electron microscopes
magnifies objects using a beam of electrons instead of light and have higher magnification and resolution
surface
scanning electron microscopes:
internal
transmission electron microscopes:
cell fractionation
takes cells apart and separates the major organelles from one another
centrifuges
fractionate cells into their component parts (differential centrifugation)
biochemistry and cytology
fields of study that correlate cell function with structure
prokaryotic cell
DNA is not enclosed within a membrane
eukaryotic cell
most of the cell’s DNA is located within the nucleus
bacteria and archaea
prokaryotic cell organisms:
protists, fungi, animals, plants
eukaryotic cell organisms:
plasma membrane, cytosol, chromosomes, ribosomes
organelles that all cells share:
plasma membrane
barrier that encloses the cell and regulates the passage of molecules in and out the cell
cytosol/cytoplasm
a jelly-like semifluid that holds the components of the cell
chromosomes
threadlike structure composed of protein and genes
ribosomes
particles composed of RNA and associated proteins found in the cytoplasm that create proteins
smaller
prokaryotes are (smaller/bigger) than eukaryotes
nucleoid
prokaryotes do not have a nucleus, but DNA in an unbound region called the:
plasma membrane
in prokaryotes, the cytoplasm is bound only by the:
double membrane
eukaryotes have DNA in a nucleus bounded by a:
membrane-bound
the organelles of eukaryotic cells are
nucleus
contains most of the DNA (genes) in a eukaryotic cell
ribosomes
use the information from the DNA to make proteins
nuclear envelope
double membrane consisting of a lipid bilayer that encloses the nucleus
pores/pore complex
regulate the entry and exit of molecules from the nucleus, and contain a structure called
nuclear lamina
within the nuclear envelope is lining called:, which is composed of proteins and maintains the shape of the nucleus
chromatin
a chromosome contains one DNA molecule associated with proteins, called:
ribosomal RNA (rRNA) synthesis
the nucleolus is the site of:
cytosol
free ribosomes build proteins in the:
on the outside of the endoplasmic reticulum/the nuclear envelope
bound ribosomes build proteins:
endomembrane system
various membrane-bound organelles in eukaryotes that modify, package, and transport proteins and lipids
smooth endoplasmic reticulum
synthesizes lipids, metabolizes carbs, detoxifies drugs and poisons, and stores calcium ions
testes/ovaries
cells that synthesize and secrete the hormones listed are rich in smooth ER
liver cells
enzymes that help detoxify drugs and poisons are found in
rough endoplasmic reticulum
many cells secrete proteins produced by ribosomes attached to the:
insulin
pancreatic cells synthesize __________ later secreted into the bloodstream
ER lumen
a new polypeptide folds into its 3D shape as it enters the
glycoproteins
secretory proteins are mostly ______________, proteins with carbohydrates
ER lumen
the carbohydrates are attached to the proteins in the
vesicles
after secretory proteins are formed, the proteins depart from the ER in:
Golgi apparatus
consists of flattened membranous sacs called cisternae
transport to the Golgi apparatus
proteins that are made in the ER:
modifies products of the ER, manufactures certain macromolecules, sorts and packages materials into transport vesicles
functions of the Golgi apparatus:
cis face
“receiving” side of Golgi apparatus
trans face
“shipping” side of Golgi apparatus
lysosomes
membranous sacs of hydrolytic enzymes that can digest macromolecules
acidic
lysosomes work best in an ___________ environment
rough ER
hydrolytic enzymes and lysosomal membranes are made by ________ and then transferred to the Golgi apparatus for further processing
trans face
some lysosomes arise by budding from this face of the Golgi apparatus
phagocytosis
when a cell engulfs another cell, forming a food vacuole
autophagy
the process of lysosomes using enzymes to recycle the cell’s own organelles and macromolecules
vacuoles
large vesicles derived from the endoplasmic reticulum and Golgi apparatus
selective
vacuolar membranes are ____________ in transporting solutes. therefore, the inside of the vacuole differs from the cytosol
food vacuoles
are formed by phagocytosis
contractile vacuoles
found in many freshwater protists and pump excess water out of cells
hydrolytic enzymes
in plants and fungi, vacuoles have:
important organic compounds
in plants, small vacuoles can hold reserves of:
poisonous/unpalatable
some protect the plants against herbivores by storing compounds that are:
pollinating insects to flowers
some plant vacuoles contain pigments, such as the red and blue pigments of petals that attract:
central vacuole
the plant cell’s main repository of inorganic ions such as potassium and chloride
growth
the central vacuole plays a major role in the _________ of plant cells
mitochondria
the site of cellular respiration, the metabolic process that uses oxygen to generate ATP
cristae
mitochondria have a smooth outer membrane and an inner membrane folded into:
intermembrane space and mitochondrial matrix
the inner membrane creates two compartments:
mitochondrial DNA and ribosomes
the matrix contains many different enzymes as well as the:
catalyzed
some metabolic steps of cellular respiration are ______________ in the mitochondrial matrix
large surface area
the highly folded surfaces of the cristae give the inner mitochondrial membrane a:
chloroplasts
found in plants and algae, and are the sites of photosynthesis
chlorophyll
chloroplasts contain the green pigment ________________, as well as enzymes and other molecules that function in photosynthesis
thylakoids
membranous sacs in chloroplasts, stacked to form a granum
stroma
internal fluid in chloroplasts
plastids
the group of plant organelles that chloroplasts belong in
endosymbiotic theory
mitochondria and chloroplasts have similarities with bacteria
endosymbiont
an early ancestor of eukaryotes engulfed an oxygen-using non-photosynthetic prokaryotic cell. the engulfed cell formed a relationship with the host cell, becoming an _______________
mitochondria
endosymbionts evolved into:
chloroplast
at least one of these cells may have then taken up a photosynthetic prokaryote, which evolved into a:
both enveloped by a double membrane, contain free ribosomes and circular DNA molecules, and grow and reproduce somewhat independently in cells
the three similarities between mitochondria and chloroplasts that support this theory:
oxidative
peroxisomes are ___________ organelles, unknown to how they are related to other organelles
peroxisomes
specialized metabolic compartments bounded by a single membrane
remove hydrogen atoms and transfer them to oxygen
peroxisomes contain enzymes that:
hydrogen peroxide (H2O2), which is toxic but later converted to water
peroxisomes form:
break fatty acids into smaller molecules for respiration
some peroxisomes use oxygen to
detoxify alcohol and other harmful compounds
in the liver, peroxisomes:
glyoxysomes
found in the fat-storing tissues of plant seeds and are used to convert fatty acids to sugar to feed the emerging seedling
endosymbiotic evolution
many peroxisomes increase in number by splitting after reaching a certain size which can indicate:
cytoskeleton
a network of fibers that helps to support the cell and maintain its shape