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56 Terms

1
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what is biotechnology?

study and manipulation of living things or their components (molecules, cells, tissues, organs, etc.) in order to benefit human beings

2
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what is classical biotech?

using living organisms to yield new products or modify foods/other useful products for human use (ex. recombinant DNA, tissue culture, fermentation, etc.)

3
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what is modern biotech?

advancements in classical biotech impacted by developments in the fields of bio, chem, genetics, & microbiology (ex. DNA profiling, genome analysis, DNA cloning, tissue engineering, etc.)

4
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what is the main difference between classical and modern biotech?

traditional: conventional use of living organisms; primarily in agronomy and consumable production

modern: focus on genetic material manipulation; extensive use in medicine, healthcare, & the environment

5
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what is domestication?

process of adapting wild plants and animals for human use; primarily through selective breeding

6
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give examples of domestication in plants/animals.

animals: dogs, cows, horses

plants: corn, wheat, apples

7
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what is fermentation?

metabolic process that produces chemical changes in organic substances through the action of enzymes; using microorganisms such as yeast or bacteria to other foods

8
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give examples of food products produced through fermentation.

booze (ex. wine, beer, and spirits), bread, cheese, pickles, & yogurt

9
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what are the 3 main modern biotech techniques?

  • directly using cells (ex. placing yeast into a bioreactor to ferment grapes)

  • using proteins/enzymes made by cells (ex. isolating antibiotics from bacteria for use in human medicine)

  • using genetic material inside cell (ex. DNA fingerprinting)

10
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what is gene therapy?

technique that modifies a person's genes to treat or cure disease

11
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what is genetic counseling?

the giving of advice to people concerning the chances of genetic disorders in a future child or themselves

12
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what is pharmacogenetics?

the study of how our genes affect the way we respond to medications

13
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what is the difference between between gene therapy, genetic counseling, and pharmacogenetics?

gene therapy aims to treat genetic disorders by modifying genes; genetic counseling provides information on hereditary risks; pharmacogenetics tailors drug treatments based on an individual's genetic makeup

14
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what is cloning?

somatic cell nuclear transfer; creating a genetically identical copy of something

15
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how is cloning a whole organism similar & different from cloning a single gene?

single genes & DNA are fairly easy to clone, but cloning entire organisms becomes increasingly difficult; entire organism: duplicating the entire DNA of the organism and creating a new individual with the same genetic makeup as the parent; gene: cloning a gene refers to the process of isolating and making copies of a specific gene

16
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what is PCR and its purpose?

polymerase chain reaction; lab technique for rapidly producing (amplifying) millions to billions of copies of a specific segment of DNA, which can then be studied in greater detail

17
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what is gel electrophoresis and its purpose?

lab technique that separates charged molecules like DNA according to size; allows for DNA sample to be easily processed and recovered without causing harm to it

18
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what is in-vitro fertilization (IVF)?

type of fertility treatment where eggs are combined with sperm outside of your body in a lab

19
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how is cloning a whole organism different than IVF?

cloning creates genetically identical organisms by transferring the nucleus of a somatic cell; IVF combines genetic material from egg and sperm donors to create a genetically unique organism

20
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how do you make rDNA with restriction enzymes?

restriction enzymes recognize short DNA sequences and cleave double-stranded DNA at specific sites within or adjacent to these sequences; DNA ligase combines the fragments made and a “vector” (ex. plasmid) can be used to insert a new segment of DNA

21
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how do you make rDNA with CRISPR Cas-9?

design guide RNA sequences for target DNA modifications; combine Cas-9 enzyme with designed gRNA to create CRISPR-Cas9 complex (Cas-( breaks double strand at target sites); deliver CRISPR-Cas9 complex into target cells using methods like transfection or electroporation

22
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how is making rDNA with restriction enzymes similar & different to than with CRISPR Cas-9?

similarities: both methods can be used to insert new genetic material into a host organism

differences:

  • restriction enzymes: cut DNA at specific recognition sites and is less precise; requires compatible ends for ligation; limited control over site specificity

  • CRISPR: uses Cas-9 enzyme guided by gRNA to induce targeted DNA breaks; often considered more versatile and precise

23
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what is industrial/environmental biotech?

  • fermented foods/beverages

  • genetically engineered proteins for industry

  • DNA identification/fingerprinting of endangered species

  • biocatalysts

  • biopolymers

24
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what is medical/pharmaceutical biotech?

  • medicines from plants, animals & fungi

  • medicines from genetically engineered cells

  • monoclonal & polyclonal antibodies

  • designer drugs & antidotes

  • prosthetics & artificial organs/tissues

  • vaccine & gene therapy

25
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what is agricultural biotech?

  • breeding of livestock/plant crops

  • aquaculture & marine biotech

  • transgenic plants/animals

  • pharmaceuticals in genetically engineered plant crops

26
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what is diagnostic research biotech?

  • DNA & protein synthesis/sequencing

  • DNA fingerprinting

  • PCR

  • genetic testing/screening

  • bioinformatics

27
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why are HeLa cells considered controversial?

original biopsy patient never consented to the use of her retrieved cells for research purposes; medicine using black women for their own benefit

28
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what was the diamond v. chakrabarty case and its significance for biotechnology?

dispute over the patentability of living organisms, particularly chakrabarty's invention; court stated that living organisms (including GMOs) are patentable; established a framework for patent protection in biotechnology

29
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how does the educational requirement vary between technical roles (e.g., laboratory technicians) and more advanced positions (e.g., biotechnologists or researchers)?

more advanced roles require more education

30
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how are the requirements for a professional degree similar & different to requirements of a graduate degree?

similarities: advanced education beyond a bachelor's degree; credentialing for higher expertise

differences:

  • professional degrees are career-focused for specific professions; graduate degrees emphasize research, theory, and varied career paths

  • professional degrees: may not require a thesis; emphasis on practical experience; graduate degrees must include research-intensive components

31
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what do each of the colors on the NFPA diamond stand for?

white: special hazard; red: flammability; yellow reactivity; blue: health hazard

32
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what is informed consent?

voluntary agreement to participate in research or medical activities after receiving detailed information about the nature, risks, benefits, & alternatives

33
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how do researchers obtain informed consent?

  • explain study details, risks, and benefits clearly

  • allow participants to ask and address concerns

  • use a written document & provide a copy to participants

34
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how can differences in enzyme function can affect a person’s response to a drug?

  • genetic differences affect the speed of drug metabolism

  • enzymatic variations impact the conversion of inactive drugs to active forms

  • enzymes influence drug absorption and availability in the bloodstream

35
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what are pre-clinical trials? which “phase” is it?

phase 0; drug is tested on human cells/tissues

36
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what is phase 1 of clinical trials?

researchers test new treatment in a small group of people to evaluate safety, dosage, and to identify side effects

37
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what is phase 2 of clinical trials?

product is given to a slightly larger group to test effectiveness and safety

38
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what is phase 3 of clinical trials?

the product is tested in a large group and compared to other drugs/treatments; used to confirm safety and monitor side effects in large populations

39
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what are post-clinical trials? which “phase” is it?

phase 4; studies conducted after clinical trials to monitor long-term safety, effectiveness, and real-world impact of medical interventions

40
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how do the conditions of clinical trials change between phases?

  • population size increases

  • earliest trials look at whether a drug is safe or the side effects it causes; later trials aim to test whether a new treatment is better than existing treatments

41
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what is OSHA and its purpose?

occupational safety and health administration; ensures workplace safety by enforcing standards for hazard communication, personal protective equipment, & emergency preparedness

42
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what is the FDA and its purpose?

food and drug administration; ensures public health by regulating safety and efficacy of food, pharmaceuticals, vaccines, & medical devices

43
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what is the EPA and its purpose?

environmental protection agency; safeguards human health and the environment by enforcing regulations, conducting research, & developing policies to prevent pollution and address environmental issues

44
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what is USDA and its purpose?

united states department of agriculture; oversees agriculture, food safety, & rural development to promote production, ensure safety, & protect natural resources

45
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what is an IRB and its purpose?

institutional review boards; provide protection for human research participants through advance and periodic independent review of the ethical acceptability of proposals for human research

46
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what is IACUC and its purpose?

institutional animal care & use committee; ensures the highest animal welfare standards and accurate scientific research in projects involving vertebrate animal testing through supervision, coordination, training, guidance, and review

47
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<p>identify the safety pictogram.</p>

identify the safety pictogram.

acute toxicity

48
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<p>identify the safety pictogram.</p>

identify the safety pictogram.

irritant

49
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<p>identify the safety pictogram.</p>

identify the safety pictogram.

flammable

50
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<p>identify the safety pictogram.</p>

identify the safety pictogram.

corrosive

51
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<p>identify the safety pictogram.</p>

identify the safety pictogram.

oxidizer

52
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<p>identify the safety pictogram.</p>

identify the safety pictogram.

health hazard

53
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<p>identify the safety pictogram.</p>

identify the safety pictogram.

environmental hazard

54
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<p>identify the safety pictogram.</p>

identify the safety pictogram.

gas under compression

55
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what are right to know laws?

employers must provide workers with basic information about the hazardous materials with which they work

56
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describe the rights/responsibilities of employers towards their employees and the communities within which they operate as defined by the right-to-know laws.

employers must provide a safe and healthy workplace, ensure equal employment opportunities, inform employees about workplace hazards (RTK laws), and minimize their environmental impact