experiment 11 | gelatin hydrolysis

define deamination and decarboxylation, understand the conditions under which each reaction usually occurs. Interpret a gelatin hydrolysis test, urea broth test, phenylalanine slant test, ornithine decarboxylase broth test, SIM deep test and understand what a positive result means and looks like

What type of macromolecule is gelatin?

Gelatin is a protein that is commonly found in certain kinds of foods.

What is the main purpose of the gelatin hydrolysis test?(exoe_ ge_)

To differentiate between organisms that produce the exoenzyme gelatinase and those that do not

What is gelatinase, and what does it help in?(pat_ ability to in_ hu_ ti_)

A pathogens ability to invade human tissues

How does gelatinase help invade human tissues?

since the gelatin molecule is too large to enter the cell the exoenzyme is secreted by gelatinase + bacteria to cut gelatin into smaller, usable polypeptides

When gelatin is converted to smaller units after the enzyme has done its job what happens to its form?

It changes to a liquid form, loss of solid consistency this is what were analyzing for

Once the media has been liquefied by the enzyme gelatinase, what will not happen again?

It will not re-solidify

What does a positive and negative result look like for a gelatin hydrolysis test?

A positive result will remain a liquid at a temperature below 32 degrees celsius and a negative result will be solid at or below 32 degrees celsius