Studied by 95 people
Blood agar results
beta hemolysis: complete hemolysis (has enzyme hemolysin)
Blood agar results
alpha hemolysis: partial hemolysis
Blood agar results
gamma hemolysis: no hemolysis (does not have enzyme hemolysis)
Mannitol Salt Agar (MSA) results
top: positive for salt tolerance, but negative for fermentation of mannitol
bottom: positive for salt tolerance AND ferments mannitol
Nutrient agar (left) and Phenylethyl Alcohol agar (PEA)
PEA: if growth, bacteria is gram (+)
if no growth, bacteria is gram (-)
Eosin Methylene Blue Agar (EMB) results
Positive for vigorous lactose fermentation
(will have green shine)
Eosin Methylene Blue Agar (EMB) results
little lactose fermentation lactose (will appear purple)
Eosin Methylene Blue Agar (EMB) results
non-lactose fermentation
(normal color)
Catalase test
negative (bacteria does not have catalase)
Catalase
positive (bacteria has catalase)
coagulase test
negative (bacteria does not have coagulase)
coagulase test
positive ( bacteria does have coagulase)
Phenol red broth
negative for both acid and gas production
Phenol red broth results
acid (+) [bc yellow]
gas (-) [bc no gas in durham tube]
Phenol red broth results
acid (+) [bc yellow]
gas (+) [bc gas in durham tube]
Phenol Red Broth
acid (+)
gas (+)
but!!! left incubated for longer than 24hrs so bacteria started using protein as energy source instead of carbohydrates
Simmonās carbon result
left: (+) for enzyme citrase (can use citrate)
right: (-) for enzyme citrase (cannot use citrate)
Triple Sugar Iron Agar (TSIA)
red slant (Absence of carbohydrate fermentation)
red butt (Absence of carbohydrate fermentation)
no gas formation
no hydrogen sulfide (H2S)
Triple Sugar Iron Agar (TSIA)
red slant (dextrose fermentation only)
yellow butt (dextrose fermentation only)
(-) gas production
No hydrogen sulfide (H2S)
Triple Sugar Iron Agar (TSIA)
Yellow slant (fermentation of dextrose, lactose and/or sucrose)
yellow butt (fermentation of dextrose, lactose and/or sucrose)
(+) gas formation (cracks)
(-) for hydrogen sulfide (H2S) (not black media)
Triple Sugar Iron Agar (TSIA)
yellow slant (fermentation of dextrose, lactose and/or sucrose)
black butt
(+) for gas (cracks)
(+) for hydrogen sulfide (black media)
Triple Sugar Iron Agar (TSIA)
red slant (dextrose fermentation only.)
black butt
(+) for gas formation
(+) for hydrogen sulfide (H2S)
Bile Esculine Agar (BEA) results
(-) for esculinase in bacteria
Bile Esculine Agar (BEA) results
(+) for esculinase in bacteria // meaning will hydrolyze esculine in bile
Sulfur reaction results
(+) for hydrogen sulfide (breaks down sulfide)
indol reaction results
(+) for indol (bacteria has tryptophanase)
Motility Reaction results
(+) for motility \\ hazy, turbid looking
Motility Reaction results
(-) motility, bacteria is only grown where stab line is
Sulfur Reduction Indol Production Motility
(-) sulfur
(+) Indol (red color on top due to kovac reagent))
(+) motility
Sulfur Reduction Indol Production Motility
(-) sulfur
(-) Indol
(+) motility
Sulfur Reduction Indol Production Motility
(+) sulfur (black media)
(-) Indol
(+) motility (assume since it is black)
Urea results
(-) does not have urease
Urea results
(+) has urease, (urea break down causes alkaine enviorment )
Note 
Flashcard (22)