ANS 124 LAB: Bioinformatics/Genbank

Between all the milk proteins we looked at in lab, did they have the same or different START codons?

same start codon ATG

Between all the milk proteins we looked at in lab, did they have the same or different STOP codons?

No, different stop codons (TAA, TGA, TAG)

What AA does the start codon ATG code for?

Met

The four forms of casein genes are found on which chromosome(s)?

All four genes are found on chromosome 6.

Are the casein genes transcribed together or at different times? Their transcription is regulated by what?

Transcribed together at the same time. Regulated by STAT5.

What is the theory behind why all four casein genes are together on chromosome 6?

There was a duplication event of one original casein gene that led to 4 separate casein genes across evolution.

What type of trait/locus are the casein genes?

Quantitative Trait Locus (QTL)

Since casein genes are a QTL, what does that mean we can select for in our animals?

Select animals for casein production.

What strategy/tool is used to compare a genetic sequence you have to a database of genetic sequences to see if there's a match or any similar sequences?

BLAST (Basic Local Alignment Search Tool)

Once you know the size of the specific milk protein, what can you analyze in the SDS-PAGE gel that we did last week in lab?

We can figure out which protein is at which band by using the ladder to see the size of the protein at the band.