6: Enzymes Equations

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What is Vo in terms of Vmax?

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What is Vo in terms of Vmax?

Vo = Vmax*[S] / (Km + [S])

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What is Vo in terms of kcat?

Vo = kcat*[Et][S] / (Km + [S])

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3
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What is kcat equal to?

kcat = Vmax / [Et]

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What is Vo during competitive inhibition?

Vo = Vmax*[S] / (αKm+[S]) OR Vo = kcat*[Et][S] / (αKm + [S])

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5
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What is Vo during uncompetitive inhibition?

Vo = Vmax*[S] / (Km + α'[S]

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6
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What is α equal to?

1 + [i]/Ki where i is the inhibitor

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7
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What is a' equal to?

1 + [i]/K'i where i is the inhibitor

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8
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What is Ki equal to?

Ki = [E][I] / [EI]

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9
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What is Ki' equal to?

Ki' = [ES][I] / [ESI]

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