Physiology: Lab Practical #1

Express numbers in the form of scientific notation: 1760

1.76 × 10³

Express numbers in the form of scientific notation: 2,200,000

2.2 × 10^6

Express numbers in the form of scientific notation: 0.00064

6.4 × 10^-4

Express numbers in the form of scientific notation: 304,000

3.04 × 10^5

Express numbers in the form of scientific notation: 0.00003

3 × 10^-5

Express numbers in the form of scientific notation: 43.125

4.3125 × 10¹

Express numbers in the form of scientific notation: 0.0056

5.6 × 10^-3

Express numbers in the form of scientific notation: 0.0000073

7.3 × 10^-3

Express numbers in the form of scientific notation: 2.750

2.75 × 10^-3

Express numbers in the form of scientific notation: 4.300

4.3 × 10³

Express numbers in the form of scientific notation: 0.02

2 × 10^-2

Express numbers in the form of scientific notation: 0.0000535

5.35 × 10^-5

Express numbers in the form of scientific notation: 0.00234

2.34 × 10^-3

Express numbers in the form of scientific notation: 8900

8.9 ×10³

Express numbers in the form of scientific notation: 5.600000

5.6 × 10^6

Abbreviation for decimeter

dm

Abbreviation for milimeter

mm

Abbreviation for nanometer

nm

Abbreviation for miligram

mg

Abbreviation for deciliter

dL

Abbreviation for micrometer

um

Abbreviation for centimeter

cm

Abbreviation for gram

g

Abbreviation for Angstrom

Å

Abbreviation for cubic centimeter

cm³/cc

Express the following numbers exponentially: 0.0000034

3.4 × 10^-6

Express the following numbers exponentially: 72.00000

7.2 × 10^7

Express the following numbers exponentially: 6050

6.05 ×10³

Express the following numbers exponentially: 0.00348

3.48 × 10^-3

Convert: 3.65 grams to milligrams

3650mg

What is the name for the Red objective lens: 4x

Scanning power

What is the name for the Yellow objective lens: 10x

Low power

What is the name for the Blue objective lens: 40x

High power

What is the name for the White objective lens: 10x

Oil Immersion

What is the micrometers for the 100x total magnification of view?

1600 uq

What are the monomers that construct proteins

Amino Acids

What are the monomers that construct carbohydrates

Glycogen

What are the monomers that construct nucleic acids

Nucleotides

Carbohydrates are under what test(s)?

Benedict’s and Lugol

Lipids are under what test?

Grease

Proteins are under what test?

Biuret and Ninhydren

Beer’s Law states…

The absorptive capacity of a dissolved substance is directly proportional to its concentration in a solution.

Transmittance def

How much light is going through.

Absorbance def

How much light is being blocked.

Spectrophotometer def

To determine light absorbance measurement.

How to calculate protein concentration

Volume of Albumin x 2.5 mg/mL = answer mg of protein / 8 = protein concentration.

Balanced equation

2H2O2

Net diffusion

Uniformed distribution of solute molecules.

Net Osmosis

Net movement of water molecules from a region of high water concentration to a region of low water concentration.

Osmotic Pressure

Concentration of dissolved particles.

Diffusion

The spreading of molecules

Osmosis

Movement of water from high concentration to low concentration.

Brownian Movement

Random movement of solutes in a solution.

Tonicity

Concentration of a solution as compared to another solution.

Isotonic

Solution having equal osmotic pressures.

Hypertonic

Solution with a high osmotic pressure.

Hypotonic

Solution with a low osmotic pressure.

Understand what occurred in sheep blood cell experiment.

Hemolysis and crenation occurred. We aw the isotonic, hypertonic, and hypotonic measures in the blood.

Understand the purpose behind the great osmosis race experiment.

To see the movement of water from high concentration to low.

What is #1?

Ocular Lens

What is #2?

Nosepiece

What is #3?

Objective lenses

What is #4?

Stage

What is #5?

Iris Diaphragm

What is #6?

Condeser

What is #7?

Light source

What is #8?

Base

What is #9?

Mechanical Stage Knobs

What is #10?

Fine Focus Knob

What is #11?

Coarse Focus Knob

What is #12?

Stage Clips

What is #13?

Arm

What is #14?

Body Tube

Monomers that construct: Proteins

Amino Acids

Monomers that construct: Carbohydrates

Glycogen

Monomers that construct: Nucleic Acids

Nucleotides

What were the four tests that was applied carbon compounds?

Benedict, Lugol, Grease, and Biuret.

What did Lugol’s test determine?

Complex Carbohydrate

What did Benedict’s test determine?

Simple Sugars

What did Grease’s test determine?

Lipids

What did Biuret’s test determine?

Proteins

The 3 rules that govern the rate of diffusion

1. The greater the concentration difference, the faster the rate of diffusion. 2. The high the temperature the greater the rate of diffusion. 3. The lighter the molecule, the greater the rate of diffusion.

What does transmittance mean in terms of a spectrometer?

How much light is going through.

What does absorbance mean in terms of a spectrometer?

How much light is being blocked.

What are the 6 receptors

1. Photoreceptors 2. Mechanoreceptors 3. Thermoreceptors 4. Osmoreceptors 5. Chemoreceptors 6. Nociceptors.

1. Photoreceptors

Responsive to light.

2. Mechanoreceptors

Sensitive, tendons and muscles. Stretch.

3. Thermoreceptors

Sensitive to heat and cold.

4. Osmoreceptors

Detects increase of salt concentration.

5. Chemoreceptors

Sensitive to specific chemicals. In the taste buds and nose.

6. Nociceptors

Pain receptors. In the skin.

Two receptors we looked at

Pacinian Corpuscle and Meissner’s Corpuscle

Pacinian Corpuscle

Deep pressure. Located in Subcutaneous tissue

Meissner’s Corpuscle

Light touch. Located in the Dermal Papillae.

Referred pain experiment

Elbow in ice. Pain felt at the fingers because that’s where the pain receptors are located.

Taste buds experiment (4)

1. Bitter 2. Sour 3. Salty 4. Sweet

Accommodation problems

1. Emmetropia 2. Myopia 3. Hyperopia 4. Nystagmus

1. Emmetropia

Normal shaped eye

2. Myopia

Eye too long (nearsightness)

3. Hyperopia

Eye shape too short (Farsightness)