Culture Media

Chocolate agar

Enriched medium for Haemophilus and Neisseria

Strep Selective Agar (SSA)

Selective medium for S. pyogenes and S. agalactiae

• MacConkey agar (MAC)

• Eosin Methylene Blue (EMB)

Selective differential media for isolation of enteric gram-negative rods

Sorbitol MacConkey (SMAC) agar

Selective medium for E. coli O157:H7

• Modified Thayer Martin

• Martin-Lewis

• NYC medium

Selective enrichment media for recovery of Neisseria gonorrhoeae and Neisseria meningitidis from specimens with normal flora

Cycloserine cefoxitin fructose egg yolk agar (CCFA)

Selective medium for Clostridium difficile

Egg yolk agar

Medium for determination of lecithinase and lipase production by clostridia and fusobacteria

Laked kanamycin-vancomycin blood agar (LKV)

Selective medium for Bacteroides and Prevotella

Thioglycollate broth (THIO)

• All-purpose medium that supports the growth of most aerobes and anaerobes.

• Can be used as a backup broth to detect organisms present in small numbers or anaerobes.

• Cystine-tellurite blood agar (CTBA)

• Tinsdale agar

Selective, differential media for isolation of Corynebacterium diphtheria

Cefsulodin-Irgasan-Novobiocin agar (CIN)

The selective medium recommended for the isolation of all three bacteria—Yersinia enterocolitica, Aeromonas spp., and Plesiomonas shigelloides is:

Alkaline Peptone Water (APW)

The primary enrichment medium for the recovery of Vibrio and Aeromonas from stool samples is:

TCBS agar

The primary selective medium for Vibrio is:

Buffered charcoal yeast extract agar (BCYE)

Enrichment medium for isolation of Legionella

Lowenstein-Jensen (LJ) medium

The most common selective enrichment medium for culturing mycobacteria is: