Blood bank Storage temps

All red blood cell products

1-6C

Transport 1-10C

Frozen red blood cells

< -65C

Transport Maintain frozen state

Platelet products

20-24C with continuous agitation

Transport maximum time without agitation is 30 minutes

Frozen plasma products / CRYO

< -18C

Transport maintain frozen state

Cold Stored platelets

1-6C with optional agitation

Transport 1-10C

All thawed plasma products

1-6C

Transport 1-10C

Thawed Cryo AHF

Apheresis granulocytes

20-24C

Transport As close as possible to 20-24C

Purpose of blood storage

Viability/Stability

Inhibit growth

Prevent clotting

Maintain normal hemoglobin function

Oxygen dissociation shift to the right

Increased 2,3 DPG has a lower affinity for oxygen

Oxygen dissociation shift to the left

Decreased 2,3 DPG has a higher affinity for oxygen

2,3 DPG levels in blood storage

decrease in stored blood with first 10 days of storage

Requires 24 hours to return to normal

RBC lesion of storage

Decreased

• pH

• ATP

• 2,3 DPG

Increased

• Na+ / K+

  • Plasma hemoglobin

RBC storage additives

Improve ATP levels

Saline, dextrose, and adenine

CPD/CP2D

CPDA-1

AS-1 —> 7

Within 72 hours of phlebotomy

Blood collected in CPD or CP2D

plasma removed, approved for 21 days storage, 1-6C

Blood collected in CPDA-1

Approved for 35 days storage, 1-6C

AS-1, AS-3, AS-5, AS-7

Contains mannitol, saline, adenine, dextrose

Storage up to 42 days, minimize hemolysis

Rejuvenation solutions

added 3 days after expiration

Increase levels of 2,3 DPG and ATP

Infused within 24 hours or frozen

Rejuvenation solutions contents

Pyruvate, inosine, phosphate, adenine, and glucose