(3) BLOOD CULTURE COLLECTION

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23 Terms

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Venipuncture

Collection Method (blood culture):

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Microbiology

What section does blood culture belong?

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70% alcohol & 2% Iodine for 1 minute prior to collection

Skin Disinfection (blood culture):

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three changes of alcohol-iodine

Disinfecting the sites (blood culture) with _______.

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fever spike

Best Drawing Time for Blood Culture Collection:

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It is because the bacteria concentration is very high which results in the multiplication of the bacteria

Why fever spike is the best time to draw or collect sample?

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2 – 3 different sites w/n 24 hrs

Frequency (blood culture):

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10 – 20 mL

Amount for Adults (blood culture):

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1 – 5 mL

Amount for Infants and small children (blood culture):

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1:10

Blood: Broth Dilution (blood culture)=

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ETS luer

- it is the portion of the butterfly device that allows you to collect multiple tubes with unlimited amount of sample

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Sodium polyanethole sulfonate (SPS)

Anticoagulant of Choice for Blood Culture?

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prevents phagocytosis, inactivates complement, and neutralizes aminoglycoside antibiotics

What is the action of SPS?

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May inhibit growth of:
â–Ş Neisseria
â–Ş Gardnerella vaginalis
â–Ş Streptobacillus moniliformis

What are the disadvantages of SPS?

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EDTA, Heparin, and Citrate

Blood culture is not recommended for:

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Nutrient Broth or BHIB

Primary Inoculation Medium (Blood Culture):

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Brain Heart Infusion Broth

BHIB means?

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ARD or resin bottles may be used

____________ = if patient is on antibiotic treatment

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Antibiotic removing devices

ARD means?

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Membrane attack complex

MAC

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Membrane attack complex

- it creates a pore or hole on the membrane

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bacT/ALERT

- allow to incubate culture bottle after collection
- allow the grow of macrobacteria

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Blood Bank

3 changes of alcohol and iodine is also seen in _______.