(3) BLOOD CULTURE COLLECTION

Venipuncture

Venipuncture

Collection Method (blood culture):

Microbiology

What section does blood culture belong?

70% alcohol & 2% Iodine for 1 minute prior to collection

Skin Disinfection (blood culture):

three changes of alcohol-iodine

Disinfecting the sites (blood culture) with _______.

fever spike

Best Drawing Time for Blood Culture Collection:

It is because the bacteria concentration is very high which results in the multiplication of the bacteria

Why fever spike is the best time to draw or collect sample?

2 – 3 different sites w/n 24 hrs

Frequency (blood culture):

10 – 20 mL

Amount for Adults (blood culture):

1 – 5 mL

Amount for Infants and small children (blood culture):

1:10

Blood: Broth Dilution (blood culture)=

ETS luer

- it is the portion of the butterfly device that allows you to collect multiple tubes with unlimited amount of sample

Sodium polyanethole sulfonate (SPS)

Anticoagulant of Choice for Blood Culture?

prevents phagocytosis, inactivates complement, and neutralizes aminoglycoside antibiotics

What is the action of SPS?

May inhibit growth of:
▪ Neisseria
▪ Gardnerella vaginalis
▪ Streptobacillus moniliformis

What are the disadvantages of SPS?

EDTA, Heparin, and Citrate

Blood culture is not recommended for:

Nutrient Broth or BHIB

Primary Inoculation Medium (Blood Culture):

Brain Heart Infusion Broth

BHIB means?

ARD or resin bottles may be used

____________ = if patient is on antibiotic treatment

Antibiotic removing devices

ARD means?

Membrane attack complex

MAC

Membrane attack complex

- it creates a pore or hole on the membrane

bacT/ALERT

- allow to incubate culture bottle after collection
- allow the grow of macrobacteria

Blood Bank

3 changes of alcohol and iodine is also seen in _______.