LAHM - Exam 2 Assignments

Which animal source provides animals with a verifiable health status from birth?

Class A

Why is the health status of the animal at the time of purchase important?

You minimize the introduction of unknown or unwanted variables when you know the health status at the time of purchase.

An animal with an unknown, uncontrolled variety and number of microorganisms is called:

Conventional

Axenic is a term for an animal which has a microbiological status which is:

Germ-free

What does ‘SPF’ stand for?

Specific Pathogen Free

Which of the following outlines the regulations for transport of animals used in research?

AWA (Animal Welfare Act)

Which of the following is NOT required to be written on the cage card for the animal?

Age and weight

What is the purpose of quarantine?

Apply permanent ID (if applicable), Verify the health status, Acclimation period

Quarantine rooms should be maintained under which of the following conditions?

Negative pressure, biocontainment barriers

What is the acclimation period?

Stress recovery and adjustment to husbandry and environment.

During which of the following periods will conditioning occur?

Quarantine

The typical quarantine period for the average healthy animal is:

72 hours

The IP route means that you are injecting in which of the following locations?

Intraperitoneal

T/F: All animals must enter quarantine upon arrival to a new facility or before moving to a new study at the same facility.

True

Which of the following signs would indicate that barbering of mice is occurring?

Alopecia with healthy skin

Porphyrin staining is an indication of which of the following in rodents?

Stress, disease, or injury

The direct sentinel program places sentinel animals into which location?

A shared microenvironment with each individual animal on the study.

An acceptable method of euthanasia is one in which:

The method consistently causes rapid death.

Hypoxia causes euthanasia by which of the following methods?

Inadequate supply of oxygen to tissues and cells.

Hypoxia is NOT an appropriate method of euthanasia for which of the following?

Reptiles, Amphibians, Diving ducks, and Diving mammals

Which of the following methods of euthanasia would be BEST for a group of animals?

Gaseous agents

Adjunctive methods are those which can only be used when:

Animals are unconscious, anesthetized, or insentient.

Which route is considered adjunctive for administration of chemical agents for euthanasia?

IC

Sodium pentobarbital is the most common agent for which category of euthanasia?

Chemical agents

What is MS - 222?

A topical chemical agent used for euthanasia.

MS - 222 is commonly used for euthanasia in which species?

Amphibians and fish

Which of the following compressed gases is most commonly used for euthanasia?

Carbon dioxide

Adjunctive agents are most commonly used for which of the following purposes?

To confirm death in animals which are difficult to assess.

What is a sentinel?

Animals used to detect foreign agents in animals on a study.

T/F: Isolation is for animals which become sick while on a study.

True

T/F: It is less stressful to euthanize animals in a foreign cage that is unknown to them.

False

T/F: Filling the chamber with the gaseous agent before introducing the animal reduces stress.

False

T/F: The euthanasia procedure is NOT complete until you have verified death.

True

How should you move the decimal to convert liters to milliliters?

3 places to the right

If you have a rat weighing 550 grams, how many kilograms does this same rat weigh?

0.55 kg

How do you convert Fahrenheit to Celsius?

(F-32)÷1.8=Celsius

What is the purpose of performing a dilution?

Measure, administer, and absorb correctly for the patient.

During which step in experimental design will you state your hypothesis?

Step 2: Design the experiment.

Unwanted variables are problematic if they occur during the study because:

Data analysis may be compromised and results altered.

Your study is not complete until you perform which of the following?

Report your findings.

What is favoritism?

Bias in a study caused by handling and interacting differently with animals on the same study.

T/F: Changes or differences in husbandry or macroenvironment for animals on the same study can introduce unwanted variables.

True

What is the scientific name for the laboratory rat?

Rattus norvegicus

Which rat stock is known as the 'hooded rat'?

Long-Evans

The front teeth of rats grow continuously throughout their lives and are called:

Incisors

Rat pups are weaned at how many days of age?

20-21

What is NOT usually considered a sign of illness or distress in a rat?

Sleeping during the day.

Which of the following methods of euthanasia would not be acceptable in an adult rat weighing over 200 grams?

Cervical dislocation

Which two mating systems are commonly used in rats?

Monogamous, polygamous

Ring tail is a condition of rats that can occur when the humidity is:

Less than 20%

What is the scientific name for the Syrian hamster?

Mesocricetus auratus

What is NOT an appropriate method for handling hamsters?

Holding the base of the tail

Which antibiotic should never be used in hamsters?

Penicillin

What is a characteristic of hamsters?

They hibernate when the termperaure drops.

What is an anatomical feature of hamsters?

Expandable cheek pouches

What are genes made from?

DNA

What is the term for a fertilized ovum?

Zygote

Which of the following cells do NOT have paired chromosomes?

Ovocytes

What is the period of time where a female animal is sexually receptive?

Estrus

Breeding animals for desirable characteristics, where these animals share a common ancestor, is called?

Line breeding

What is the scientific name for the common laboratory mouse?

Mus musculus

How long after mating can mucous plugs remain in the rodent's vagina?

24 hours

What is the term used when a mouse chews the hair off its cage mates?

Barbering

When is toe clipping usually performed for identification?

With neonates, with IACUC approval.

How long is the gestation period in non-lactating female mice?

19 - 21 days

In addition to having no hair, what is another characteristic of nude mice?

They have a deficiency in their immune system.

Which of the following methods of euthanasia is acceptable for a mouse?

Barbiturate overdose.

Which of the following parasites can be detected using the perianal tape test?

Syphacia

Which of the following inbred strains of mice have a genetic predisposition to ulcerative dermatitis?

C57BL/6