AQA A-Level Biology: Food Tests

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Test for Starch

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  • Add 2 cm³ of sample to be tested into a clean test tube

  • Add 2 cm³ of Iodine solution

  • Positive Result: Orange to Blue-Black

  • Negative Result: Colour remains Orange

  • The test is qualitative, not quantitative.

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Test for Reducing Sugar

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  • Add 2cm³ of sample to be tested into a clean test tube

  • Add 2cm³ of Benedict’s Solution and heat sample in a water bath

  • Positive Result: Blue to Brick-Red

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8 Terms

1

Test for Starch

  • Add 2 cm³ of sample to be tested into a clean test tube

  • Add 2 cm³ of Iodine solution

  • Positive Result: Orange to Blue-Black

  • Negative Result: Colour remains Orange

  • The test is qualitative, not quantitative.

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2

Test for Reducing Sugar

  • Add 2cm³ of sample to be tested into a clean test tube

  • Add 2cm³ of Benedict’s Solution and heat sample in a water bath

  • Positive Result: Blue to Brick-Red

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3

Test for Non-Reducing Sugar

  • Add 2cm³ of sample to be tested into a clean test tube

  • Add 2cm³ of Benedict’s Solution and heat sample in a water bath

  • Negative Result: Colour remains Blue

  • Heat the sample with Dilute HCl for hydrolysis

  • Neutralise with NaOH

  • Repeat Benedicts Test whilst heating solution in a water bath

  • Positive Result: Blue to Brick-Red

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4

Test for Lipids

  • Add 2cm³ of sample to be tested into a clean test tube

  • Add 2cm³ of Ethanol to sample and shake

  • Add 2 cm³ of water to sample and shake

  • Positive Result: Clear to Milky-White emulsion

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5

Test for Protein

  • Add 2cm³ of sample to be tested into a clean test tube

  • Add an equal volume of NaOH and CuSO4 at room temp

  • OR add Biuret Reagent to sample and mix gently

  • Negative result: Colour remains Blue

  • Positive result: Blue to Purple/lilac

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6
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  • Filter and dry the precipitate

  • Find the mass/ weight

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7
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  • A = Glucose and B = Maltose;

  • Because more sugar/ precipitate after hydrolysis/ maltase action

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8
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  • Quantitive OR (colour change is) subjective

  • Standardises the method

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