Features of behaviors, morphology, or genetics which serve to prevent mating or breeding between two different species (e.g., temporal isolation, in which individuals are active at different times of the day, seasons, or mating periods; ecological isolation, in which individuals only mate in their specific habitat; behavioral isolation, when there are no sexual cues between representatives of the species; mechanical isolation, when there is no sperm transfer during an attempted mating; and gametic incompatibility, when there is sperm transfer without fertilization occurring).If mating can take place, there are four factors that prevent hybrid viability: zygotic mortality (fertilization but no zygote), hybrid inviability (embryo is not viable), hybrid sterility (resulting adult is sterile), and hybrid breakdown (first generation is viable but future generations are not).