lab practical 1

What is the correct way to open an apgar plate to either remove a sample or to inoculate?

A. Remove the lid completely, holding it in your hand, not setting it on the bench

B. Open the lid slightly on one side, in a clamshell fashion

C. Remove the lid completely and set it on the bench upside down

D. Remove the wood completely and set it on the bench and upright position

B

A substance used to support the Growth of microbial life is referred to as

A. Culture medium

B. Water

C. Incubator

D. Inoculating loop

A

Bacterial cultures are typically incubated at —— degrees

A. 0°C

B. 24°C

C. 37°C

C

Which type of media is best for observing isolated bacterial colonies

A. Agar Plate

B. Nutrient broth

C. Agar Slant

A

Which of these methods is not a procedure for an aseptic techniques?

A. Disinfect of lab bench before and after lab exercise exercises

B. Washing hands before and after lab exercise exercises

C. Sitting the tops of petri dishes and tubes on the lab bench

D. Using heat to sterilize the inoculating loops, needles, and tube media openings before and after transfer procedures

C

The goal of ____ technique is to minimize the contamination of both original annually, inoculated cultures, as well as to protect the laboratory worker from contamination

A. Sterile

B. Inoculation

C. A septic

D. Antiseptic

C

A pure culture contains

A. A variety of microbes from one source

B. Only bacteria

C. Only one species of microorganism

D. A variety of species from the same genius

C

In ___ growth, there is no separation between individual colonies, growing on a petri dish

A. Isolated

B. Confluent

C. Turbid

B

What is the name of the substance that is used to make culture media solidify?

A. agar

B. Slant

C. Seaweed

D. Trypticase

A

Media and slants and petri dishes are solidified by the addition of

A. Seaweed

B. Agar

C. Gelatin

D. Polypeptides

B

A ____ is a mound of cells on a solid medium that represents the progeny from one original bacterial cell

A. Colony

B. Culture

C. Streak

D. Quadrant

A

During the subculturing process, when is the inoculating instrument sterilized?

A. Following the inoculation is sufficient

B. It is not necessary to sterilize the inoculating instrument during subculturing

C. Prior to, and after each inoculation

D. Prior to the inoculation is sufficient

C

You have a nutrient broth, an Agar slant, in an agar plate with bacterial growth in or on each. If you wanted to evaluate the macroscopic appearance of the bacteria growing in or on the medium, which of the cultures would be the best choice.

A. Agar plate

B. Agar Slant

C. Broth

A

The waxy substance found in my bacterial cell walls is

A. My colic acid

B. Lipopolysaccharide

C. Peptidoglycan

D. Nucleic acid

A

Smears are

A. Fixed to the side by refrigeration

B. Air dried

C. Heated over a flame to dry

B

Identify the correct order for the reagent used in Graham stain procedure

A. Ethyl alcohol, crystal violet, safranin, grams iodin

B. Crystal violet, grams iodine, ethyl alcohol, safranin

C. Safranin, ethyl alcohol, crystal violet, grams iodine

D. Crystal violet, safranin, grams iodine, ethyl alcohol

B

Is the capsule stain a positive or negative staining procedure

Negative

Before you use reagents to stay in the bacteria on a slide, you heat fix your sample first. Why do you do this?

A. So that the Bacteria will not wash off

B. So that the bacteria will not die

C. So that the bacteria will be healthy and happy

D. So that the bacteria will react to the stain

A

In a ____ stain, the background around the bacteria or other cells is stained, and the other cells are unstained

A. Differential

B. Simple

C. Negative

C

Which two stains are used in the capsule stain procedure

A. Methylene blue and nigrosin

B. Nigrosin and crystal violet

C. Safranin and methylene blue

D. Malachite green and methylene blue

B

What are the two genera for which the endospore stain is used?

Myobacterium and staphylococcus

Acidic dyes

A. Contain cations and stain bacteria

B. Contain positive ions and stain bacteria

C. Contain anions and stain in the slide background

D. Reveal the presence of Myobacterium

C

The presence of Greece or dirt on a glass slide

A. Will be removed by gram Stain process

B. Is of no consequence due to simple stains

C. Will be removed by the acid fasting process

D. Results in poor smear preparation

D

The gram stain An acid fasting are based on chemical properties of the

A. Cell wall

B. Capsule

C. Plasma membrane

D. Lipopolysaccharide layer

A

The acid fasting is used to identify bacteria in which genus

A. Bacillus

B. Mycoplasma

C. Clostridium

D. Myobacterium

D

A ____ stain uses two or more dyes to differentiate between bacterial species or distinguished between structures of an organism

A. Negative

B. Differential

C. Simple

B

A __ stain consist of only one dye and is used to study shape and arrangement of bacterial cells

A. Simple

B. Differential

C. Negative

A

You were performing the Gram stain procedure on a slide, but forgot the step with Ethyl alcohol. Describe what the bacterial cells would look like under the microscope if the step was missing

A. All cells would be pink

B. All cells would be colorless

C. All cells would be purple

D. All cells would wash off the slide

C

Choose the answer that has the steps of the gram stain in the correct order

1. Ethyl alcohol 2. Crystal violet C. Safranin D. Iodine

A. 1234

B. 2413

C. 2143

D. 4321

B

Choose the answer that has the steps Of the acid fasting in the correct order

1. Acid alcohol 2. methylene blue 3. Carbon fuchsin

   A. 123

   b. 213

   c. 312

   d. 321

C

Heat fixing a smear ensures

A. That gram-negative organisms will decolorize

B. That the smear is not cold so stains will color microbes

C. That water boils creating steam

D. That bacteria adhere to the glass slide

D

Identify the correct order for the re-agent used in the endospore stain

A. Malachite green over steam, rinse with alcohol, safranin

B. Malachite green over steam, rinse with water, safranin

C. Safranin over steam, rinse with alcohol, malachite green

D. Safranin over stream, rinse with water, malachite green

B

Identify the correct order for the reagent used in the acid fast stain

A. Acid alcohol, methylene blue, carbol fuchsin

B. Methylene blue, acid alcohol, carbol fuchsin

C. Carbol fuchsin, acid alcohol, methylene blue

C

Members of the ___ genus are acid fast stain

A. Proteus

B. Myobacterium

C. Escherichia

D. Staphylococcus

B

which of the following is not part of the PPE required in a bio safety level two lab

A. Mask

B. Goggles

C. Gloves

D. Clothes, toed shoes

A

Lab coat, gloves, face, masks, and goggles are all examples of

Personal protective equipment

A disinfectant is used on your work Surface

A. After all work is complete

B. After any spill of live microorganisms

C. Before the beginning of laboratory procedure

D. All of the answer choices are correct

D

The Gaspak system creates an __ environment within

A. Anaerobic

B. Aerobic

C. Cold

D. Alkaline

A

Facultative anaerobes

A. Can grow in the absence of free oxygen, but prefer to grow with it

B. Require the absence of atmospheric oxygen for growth

C. Require limited amounts of atmospheric oxygen for growth

D. Can grow in the presence of free oxygen, but prefer to grow without it

A

The reaction of the H2 gas generated by the Gaspak enveloped and the O2 in the air in the jar forms water:

2H2+O2=2H2O

What is the catalyst for this reaction?

Palladium

An indicator strip is used to determine if the atmosphere in the anaerobic jar is anaerobic. What is the dye on the strip?

A. Crystal violet

B. Methylene blue

C. Safranin

D. Malachite green

B

An indicator strip is used to determine if the atmosphere in the anaerobic jar is anaerobic. What is the dye on the strip?

Methylene blue

E.coli will grow exposed to room air in the laboratory incubator, and also within an anaerobic culture vessel. E.coli is a

A. Facultative anaerobe

B. Aerobe

C. Anaerobe

A

A colony that grows on anaerobic plates means the colony is an obligate anaerobic

True or false

False

The indicator strip in the jar used to create an anaerobic conditions changes from blue to ____ when atmosphere becomes anaerobic

A. Green

B. Red

C. White

D. Yellow

C

Calculate the total magnification of a specimen if the ocular lens is 10x and the objective is 100x

1000x

Immersion oil is used with the 100x microscope objective because

A. It facilitates movement of the microscope mechanical stage

B. It sets the simple stain

C. It limits light scattering

D. Keeps the bacterial smear moist

C

Which category best describes Mannitol Salt Agar

A. Enriched and differential

B. Enriched

C. Selective and differential

D. Differential

E. Selective

C

Which category best describes Blood Agar

A. Differential

B. Selective and differential

C. Enriched

D. Enriched and differential

E. Selective

D

Growth and a green metallic sheen on the eosin methylene blue plate indicates

A. Gram positive cocci, glucose fermenter

B. Gram positive cocci, non glucose fermenter

C. Gram negative bacilli, non lactose fermenter

D. Gran negative bacilli, lactose fermenter

D

Which category best describes eosin methylene blue C

A. Selective and differential

B. Enriched and differential

C. Differential

D. Enriched

E. Selective

A

Which term best describes MacConkey agar

A. Enriched

B. Enriched and differential

C. Elective and differential

D. Selective

E. Differential

C

MacConkys agar is selective for

A. Acid fast bacteria

B. Gram positive cocci

C. Spore formers

D. Gram negative bacilli

D

Eosin methylene blue agar is selective for

A. Gram negative bacilli

B. Staphylococci

A

Mannitol salt agar is selective for

A. Staphylococci

B. Gram negative bacilli

A

What is the order of the tubes?

A. Obligate aerobe

B. Faculative anaerobe

C. Aerotolerant anaerobe

D. Obligate anaerobe

E. Microaerophilic

Identify the two genera for which the endospore stain is used

• Myobacterium

• Staphylococcus

• Bacillus

• Clostridium

Bacillus and Clostridium