Platelets & Plasma

Platelet Storage Conditions

20-24 C - Room Temp

Continuous Agitation

Expires 5 days post-collection

Granulocyte Transfusion Purpose

Used for Neutropenic patient transfusions to fight infections

Platelet Quality Control

Platelet count >5.5x10^10

pH >6.2

Plasma Dosage

Every mL will contain 1 IU of coagulation factors

When plasma transfusion may be used

- Massive Transfusion (lost factors to massive bleeding)

- Liver Failure (liver makes factors)

Cryoprecipitate Dosage

- Utilized for fibrinogen replacement

- For surgery/trauma a patient's fibrinogen should be 100 mg/dL

- Each donor bag contain 150 mg

- A person's plasma volume can be estimated at 30 dL

Cryoprecipitate Components of Interest

Factor V (5), Factor VIII (8), Fibrinogen

Fresh Frozen Plasma Freezing Window

8 hours

FP24 Freezing Window

24 hours

FFP

- Contains maximum levels of clotting factors, both stable & labile

- Expect 1 IU/mL

FP24

- Contains all stable clotting factors

- Reduced factors V (5) & VIII (8)

Cryoprecipitate

- often pooled into 5 donor units

- contains 80 units of Factor 8 & 150 mg Fibrinogen

- Factor 13 & vWF also in major quantity

- storage: Frozen < -18C for 1 year

- thaw at 37C, store at RT until expiration

- must be used within 4 hours for pools & 6 hours for singles

Cryo-Reduced Plasma

- left over plasma from making cryo

- contains plasma proteins & ADAMTS13

- storage: frozen < -18C for 1 year

- thaw and use like plasma

- best use is for patients with TTP

Cryo-reduced plasma is made by removing the cryoprecipitate from FFP (fresh frozen plasma). This removes:

• vWF (especially large multimers)

• Fibrinogen

• Factor VIII

• Factor XIII

• Fibronectin

What remains is plasma enriched in ADAMTS13, but depleted of high-molecular-weight vWF multimers, making it:

✅ Therapeutically useful in TTP because it replenishes ADAMTS13
❌ Without reintroducing the problematic UL-vWF multimers

Universal Plasma Donor

AB (b/c no antibodies made in the reverse type)