ENZYMOLOGY 6 ACP "ACID PHOSPHATASE"

ACP is formerly known as

ORTHOPHOSPHORIC MONOESTER PHOSPHOHYDROLASE

What pH does ACP catalyzes hydrolysis

pH 5-6

Tissue source with highest concentration

PROSTATE

Used for Forensic Clinical Chemistry for investigation of what case? and vaginal washing persist for how many days?

CASES OF RAPE - (+)ACP if semen is detected.
4 DAYS

What disease associated in thrombocytopenia due to platelet destruction

IDIOPATHIC THROMBOCYTOPENIC PURPURA

ACP is also associated with Paget's disease that is originated from

OSTEOCLAST
- NEIMANN PICK DISEASE
- GAUCHER'S DISEASE

What ACP isoenzymes has the greater mobility and termed as "Specific ACP"

PROSTATIC ACP

Chemical inhibitors used for Prostatic ACP

L-TARTRATE
- Prostatic ACP = Total ACP when inhibited by TARTRATE

Chemical inhibitors for Erythrocytic ACP isoenzyme

2% FORMALDEHYDE SOLUTION
1mM CUPIC SULFATE

ACP isoenzyme classified as "Non-Specific"

ERYTHROCYTIC ACP Isoenzymes

What special specimen consideration is needed by ACP in order to prevent loss

MUST BE ACIDIFIED (L-TARTRATE) - LOWER THAN 6.5pH
FROZEN

What sample choice for ACP

SERUM
-separated from the clot

How Erythrocyte from hemolyzed sample can affect the result

FALSE ELEVATION OF ACP

ACP activity decreases within __________ at room temp

1-2hours

ACP can be stabilize through

- ACIDIFIED can last for 2 days at room temp
- 7days at Refrigerated Temperature(4DegreesC)
- 30 days at Freezing temp -20DegreesC

Reference Value for ACP

TOTAL ACP
Adult:1.5-4.5 U/L
Children: 3.5 to 9.0
Male2.5-11.7
Female: 0.3-9.2

PROSTATIC ACP
Adult/Children: 0.5 TO 3.5 ng/mL
M:0.2-5.0 U/L
F:0.0-8.0 U/L

Elevated in patients with

PROSTATIC ADENOCARCINOMA
IDIOPATHIC THROMBOCYTOPENIC PURPURA

What method is used for continuous monitoring

BABSON and REED

What method use fluorescent to improve sensitivity

REITZ, GUILBAULT

Method that is more specific in prostatic form with 590nm absorbance

ROY
- Si HUDSON = rapid pero non specific with absorbance about 410 to read