Exercise 13: Blood Uric Acid Measurement

An end product of purine such as adenosine and guanosine metabolism

Blood Uric Acid

How much mg ofd uric acid is obtained from our daily diet?

300 mg

Part of the kidney that filters BUA

Glomerulus

Part of the kidney that reabsorbs BUA

Proximal and distal tubes

Enzyme responsible for the formation of Uric Acid

Xanthine oxidase

Term for increased BUA

Hyperuricemia

Term for decreased BUA

Hypouricemia

Enumerate the diseases associated with Hyperuricemia

Gout, Leukemia, Chronic Renal Disease, Lesch-Nyan Syndrome

Enumerate the diseases assocaited with Hypouricemia

Fanconic syndrome, Wilson's disease, Hodgkin's disease

In cases of Hyperuricemia, what are the drugs that are used for medication to lower BUA levels?

Colchitin and Allopurinol

Reference method of measuring BUA

Isotope Dilution Mass Spectrometry (IDMS)

Specimen utilized for BUA measurement

Serum

Reagent used for BUA measurement

Uricase

Commonly used method for measuring BUA

Colorimetric Endpoint Assay/ Uricase method

End color of Uricase method

Red quinoneimine dye

Relationship between color intensity and Uric acid concentration

Directly proportional

Incubation conditions for BUA measurement

10 minutes, room temperature

Absorbance used for BUA measurement

546 nm

Conversion factor for BUA measurement

0.059

Conventional unit for BUA

mg/dL

SI unit for BUA

mmol/L

Conventional unit reference range of BUA for males

3.5-7.2 mg/dL

Conventional unit reference range of BUA for females

2.6-6.0 mg/dL