Exercise 13: Blood Uric Acid Measurement

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An end product of purine such as adenosine and guanosine metabolism

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23 Terms

1

An end product of purine such as adenosine and guanosine metabolism

Blood Uric Acid

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2

How much mg ofd uric acid is obtained from our daily diet?

300 mg

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3

Part of the kidney that filters BUA

Glomerulus

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4

Part of the kidney that reabsorbs BUA

Proximal and distal tubes

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5

Enzyme responsible for the formation of Uric Acid

Xanthine oxidase

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6

Term for increased BUA

Hyperuricemia

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7

Term for decreased BUA

Hypouricemia

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8

Enumerate the diseases associated with Hyperuricemia

Gout, Leukemia, Chronic Renal Disease, Lesch-Nyan Syndrome

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9

Enumerate the diseases assocaited with Hypouricemia

Fanconic syndrome, Wilson's disease, Hodgkin's disease

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10

In cases of Hyperuricemia, what are the drugs that are used for medication to lower BUA levels?

Colchitin and Allopurinol

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11

Reference method of measuring BUA

Isotope Dilution Mass Spectrometry (IDMS)

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12

Specimen utilized for BUA measurement

Serum

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13

Reagent used for BUA measurement

Uricase

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14

Commonly used method for measuring BUA

Colorimetric Endpoint Assay/ Uricase method

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15

End color of Uricase method

Red quinoneimine dye

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16

Relationship between color intensity and Uric acid concentration

Directly proportional

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17

Incubation conditions for BUA measurement

10 minutes, room temperature

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18

Absorbance used for BUA measurement

546 nm

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19

Conversion factor for BUA measurement

0.059

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20

Conventional unit for BUA

mg/dL

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21

SI unit for BUA

mmol/L

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22

Conventional unit reference range of BUA for males

3.5-7.2 mg/dL

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23

Conventional unit reference range of BUA for females

2.6-6.0 mg/dL

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