MICRO Lab Midterm

Identify: Fungi

Rhizophus

Identify: Fungi

Penecillium

Identify: Fungi

Aspergillus niger

Identify: Fungi

Rhizophus Sporengium

Identify: Fungi

Sacrocandida; Yeast: budding

Identify: Fungi

Aspergillus niger

Identify: Fungi

Rhizophus Zygospore

Identify: Fungi

Rhizophus Sporengium

Identify: Fungi

Penicillium

Identify: Fungi

Rhizophus

Identify: Fungi

Rhizophus zygospore

Identify: Fungi

Yeast

Identify: Fungi

Aspergillus

Identify: Fungi

Penecillium

Identify: Fungi

Aspergillus

Identify: Protozoa

Plasmodium

Identify: Protozoa

Trypanosoma brucei

Identify: Protozoa

Balantidium coli

Identify: Protozoa

Giardia lamblia: Cyst

Identify: Protozoa

Balantidium coli

Identify: Protozoa

Entamoeba histolytica: trophoizote

Identify: Protozoa

Toxoplasma gondii

Identify: Protozoa

Trypanosoma brucei

Identify: Protozoa

Entamoeba histolytica

Identify: Protozoa

Balantidium coli

Identify: Protozoa

Entamoeba histolytica

Identify: Protozoa

Trichomonas vaginalis

Identify: Protozoa

Entamoeba histolytica

Identify: Protozoa

Balantidium coli: cyst

Identify: Protozoa

Giardia

Identify: Protozoa

Trichomonas vaginalis

Identify: Protozoa

Toxoplasma gondii

Identify: Protozoa

Trypanosoma brucei

Identify: Protozoa

Giardia lamblia: trophoizote

Identify: Protozoa

Giardia lamblia

Identify: Protozoa

Giardia lamblia: cyst

Identify: Protozoa

Balantidium coli

Identify: Protozoa

Giardia lamblia

How do you calculate total magnification?

Objective lens X Ocular lens (usually 10x)

Compound Light Microscope: Light source

Visible light

Compound Light Microscope: lens system

Ocular and Objective

In a ? microscope, the image from the ? lens is ? again by the ? lens

Compound microscope, objective lens, magnified again, ocular lens

What objective lens has the largest field of view?

4x; scanning lens

Does the field of view increase or decrease with magnification?

Decreases

What do you use to switch between the objective lenses?

Revolving nosepiece

What is the name of the 4X objective lens?

Scanning lens

What is the name of the 10X objective lens?

Low power lens

What is the name of the 40X objective lens?

High dry lens

What is the name of the 100X objective lens?

Oil immersion lens

Which objective lens has the smallest field of view?

100X; oil immersion

Does light increase or decrease with increased magnification?

Decreases

What is the purpose of magnification?

To enlarge an image in appearance

Identify: 1

Ocular Lens

Identify: 2

Revolving Nosepiece

Identify: 3

Objective Lens

Indentify: 4

Condenser

Indentify: 5

Iris Diaphragm

Identify: 6

Illuminator

Identify: 7

Coarse focus knob

Identify: 8

Fine focus knob

Identify: 9

Stage

Identify: 10

Light intensity knob

Define ocular lens

Re-magnify image formed by objective lens

Define objective lens

Primary lenses that magnify specimen

Define stage

Holds microscope slide in position

Define condenser

Focuses light through specimen

Define iris diaphragm

Controls amount of light entering condenser

Define illuminator

Light source

Define course focusing knob

Rough focus; only used in 4X

Define fine focusing knob

Sharp focus

Define light intensity knob

Controls the brightness of the Illuminator

What is the purpose of the blue filter in a light microscope?

Increases the resolution

The shorter the wavelength, the ? the resolution

Better

Define refractive index

Measure of light bending ability of a medium

Refractive index of oil

1.5

Refractive index of glass

1.5

Refractive index of air

1

Define resolution

Ability of lenses to distinguish two points

Shorter wavelength of light provide ? Resolution.

Greater

Define depth of field

Thickness of the specimen that isn’t focused at one time

Does depth of field increase or decrease as you increase magnification

Decrease

Why do we use oil immersion with 100 X objective lens?

The refractive index of glass and immersion oil are the same

If two points are 500 nm apart. A microscope with a revolving power of what will be able to distinguish the two points as separate?

Microscope with a resolving power of 450 nm

If two points are 500 um apart. A microscope with a revolving power of what would blur the two points into one?

Microscope with a resolving power of 950 um

What does the colored threads slide demonstrate about specimens you will be observing later in class?

Microscopes have a limited depth of field, meaning they could only focus sharply on one layer of a specimen at a time

What is the purpose of calibration of the ocular micrometer?

Used to take measurements of specimens under the microscope

Is the ocular scale known or unknown?

Unknown

Is the stage micrometer scale, known or unknown?

Known

How much is each tiny division?

Each division is 0.01 mm

How much is each bigger division?

Each division is 0.10 mm

How many um’s equal to 1mm

1000um

What is the calibration? 0.01mm=10ou

10mm=10um

10um/10ou= 1um/ou

What is the size (um) of the red blood cell at the 40 X objective lens giving the calibration of 5 um/ou

20 ou x 5 um/ou = 100 um

Define aseptic technique

Without contamination

Contamination from what?

Air, us, microbes

Identify: white arrow

Outercone

Identify: red arrow

Innercone

Hottest part of the Bunsen burner flame

Innercone

Define inoculate

Introduce a microbe into a new environment