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staphylococcus aureus
What organism is associated with Mannitol Salt Agar?

presence of salt
Why is Mannitol Salt Agar selective for Staphylococcus?
- presence of mannitol
- allows differentiation based on fermentation.
Why is Mannitol Salt Agar differential?

- skin
- nose
- fomites
What are common sources of Staphylococcus aureus?

staphylococcus epidermis
Which Staphylococcus species cannot ferment mannitol?

complete
What type of hemolysis would you expect if a sample is believed to be Staphylococcus aureus on blood agar?

streptococcus pyogenes
If a throat swab produces beta hemolysis on blood agar, what organism is most likely?

it contains 5% blood
Why is blood agar considered complex media?
incomplete hemolysis
What type of hemolysis is described for a sample believed to be Staphylococcus aureus that is seen in this photo?

no
Does Staphylococcus epidermidis hemolyze blood?

- streptococcus viridans
- streptococcus pneumoniae
If a throat sample shows alpha hemolysis, what organisms might it be?

E. coli
What organism is associated with a green sheen on EMB agar?

enteric organisms
What is EMB agar selective for?

prescence of methylene blue dye
What makes EMB agar selective for enteric organisms?

assorted enteric organsism
What types of organisms will grow on MacConkey agar?

enteric organisms
What is MacConkey agar selective for?

presence of bile salts
What makes MacConkey agar selective for enteric organisms?

fungi
What is Sab Dextrose Agar selective for?

presence of sugar and acid
Why is Sab Dextrose Agar selective for fungi?

prefer neutral or slightly alkaline conditions
Why will bacteria rarely grow on Sab Dextrose Agar?

slimy
What do yeast colonies look like on Sab Dextrose Agar?

fuzzy or hairy
What do mold colonies look like on Sabouraud Dextrose Agar?

efficacy of antibiotics and/or antiseptics/disinfectants
What is the Kirby-Bauer test used to measure?

- area of non-growth around the antimicrobial
- indicates susceptibility
What is the zone of inhibition?

millimeters
What unit is used to measure the zone of inhibition?
staphylococcus aureus
What is Chromagar MRSA II selective for?

methicillin-resistant staphylococcus aureus
What is Chromagar MRSA II differential for?

reddish or mauve pigment
What color do MRSA colonies appear on Chromagar MRSA II?
