Genetics Exam 3 Ch.11 Genomics Review

1.         What is one difference between using restriction endonucleases and mechanical shearing of DNA?

A. Restriction endonucleases digest at known specific sites while shearing occurs at random sites.

2. Which of the following is an example of a recombinant DNA molecule?

B. a genomic fragment of human DNA ligated to a bacterial plasmid vector.

3. What information cannot be determined from the DNA sequence of a cDNA clone?

B. sequence of the promoter.

1.         In Sanger sequencing, what causes DNA synthesis to terminate at a specific base?

D. nucleotide triphosphates that lack a hydroxyl at the 3' position.

5. In gel electrophoresis of DNA, fragments move at different rates because they have different

sizes

6. What enzyme is necessary for the copying of mRNA sequence into DNA during cDNA library construction?

C. Reverse transcriptase.

7. If DNA had 6 different bases with the two novel bases pairing with only each other, a restriction enzyme with a 4-base recognition sequence would be predicted to digest the DNA approximately every

125 bp

8. Why does a genomic library need more clones than what would be estimated by calculations to be complete?

A. There is a significant probability that some sequences would not be represented in a library if it was only the calculated size

9. Most polymorphisms do not result in a phenotypic difference since they are typically

C. either silent mutations or are in non-coding regions.

10. SNP is the acronym for:

B. single nucleotide polymorphism.

11. Mapping a disease gene in humans requires

B. large numbers of mapped physical markers with complete genomic coverage and small families with pedigrees and DNA sequences.

12. Which is the most frequent type of DNA polymorphism?

A. single base differences.