Biology AS- Cells and Magnification

What is the equation for magnification

Image= actual size * magnification

How do you transform from mm to micrometers

*1000

How do you transform from micrometers to nm

*1000

Explain why it is not possible to determine the identity of the structures labelled X using an optical microscope

Because an optical microscope zooms in up til micrometers, therefore there wouldnt be a fine detail

What are the 2 main types of electron microscopy

1. Scanning Electron Microscopy

2. Transmission Electron Microscopy

What are the principles of Scanning Electron Microscope

1. It uses electrons to form an image

2. Electrons that are rejected from the surface on the spectrum are detected

3. Resolution to 3-20 micrometers

What are the characteristics of Transmission Electron Microscope

1. Uses electrons to form an image

2. Magnification to 10^6

What are the differences of a light microscope and electron microscope

1. Light- resolution to 0.2 micrometer

   Electron- resolution to 0.5nm

2. Light- living tissues can be studied

   Electron- processing kills living cells

3. light- colours can be seen

   electron- colours cant be seen

4. Electrons are way more expensive

What are some limitations for electron microscopy

1. must be completed in a vacuum

2. requires complex staining process

3. images are not in true color