zoonotic diseases

which organisms are mainly known to cause zoonotic diseases

• Brucella spp.

• Francisella tularensis

• Yersinia pesitis

• Pasteurella multocida

• Streptobacillus moniliformis

what disease do Brucella spp. cause

Brucellosis

microscopic description of Brucella spp.

gram negative coccobacillus

significant Brucella lab diagnosis tests

• blood culture positive in the first 2 weeks

• hold for 21 days

what organism should be held for 21 days

Brucella

B. abortus is associated with what animal

cows

B. suis is associated with

pigs

B. melentensis is associated with

goats

B. abortus biochemical posiitve tests

• CO2

• H2S

• basic fuschin

B. abortus biochemical negative tests

• thionin

B abortus is CO2 _

positive

B. suis is CO2 _

negative

B. melentensis is CO2 _

negative

B. abortus is H2S _

positive

B. suis is H2S _

positive/negative

B. melentensis is H2S _

negative

B. abortus is thionin _

negative

B. suis is thionin _

positive

B. melentensis is thionin _

positive

B. abortus is basic fuschin _

positive

B. suis is basic fuschin _

negative

B. melentensis is basic fuschin _

positive

positive biochemical tests for B. suis

• thionin

  • maybe H2S

negative biochemical tests for B. suis

• CO2

• basic fuschin

  • maybe H2S

positive biochemical tests for B. melentensis

thionin and basic fuschin

negative biochemical tests for B. melentensis

CO2 and H2S

which zoonotic organisms are BSL 3

Francisella tularensis

Yersinia pestis

which zoonotic organisms are BSL2

Pasteurella multocida

characteristic biochemical test for Francisella tularensis

H2S positive with lead acetate

what condition does F. tularensis cause

Tularemia

tularemia is also known as

rabbit fever

what animal is Francisella tularensis associated with

rabbits

etiologic agent of rabbit fever

Francisella tularensis

what media is used for F. tularensis

cysteine-glucose media

F. tularensis microscopically

faintly staining GN coccobacillus

what do the colonies of F. tularensis look like

tiny pinpoint colonies

what is the organism that infects others with F. tularensis

tick bite

describe Yersinia pestis microscopically

GN bi polar staining

• resembles safety pin

which organism reembles a safety pin

Y. pestis

what disease does Y. pestis cause

plague

what is Y. pestis transmitted by

fleas, rats, other mammal reservoirs

describe Pasteurella multocida microscopically

GN rods with bipolar staining

biochemical tests for P. multocida

• oxidase positive

• indole positive

• ferments glucose and sucrose

which tests is P. multocida positive for

oxidase and indole

which sugars does P. multocida ferment

glucose and sucrose

P. multocida on MAC

no growth

how is P. multocida contracted

cat and dogs bites

what is a unique characteristic of P. multocida

mousy odor

how do Streptobacillus moniliformis appear microscopically

long filamentous GN rods with swellings (pleomorphic)

how does Streptobacillus moniliformis appear in thioglycolate broth

puffball or string of pearls colonies

Streptpbacillus moniliformis causes what condition

Haverhill fever / rat bite fever

haverhill fever is also known as

rat bite fever

etiologic agent of rat bite fever

Streptobacillus moniliformis

etiologic agen of haverhill fever

Streptobacillus moniliformis

what sample is NEEDED for Streptobacillus moniliformis

ascitic fluid

what inhibits the growth of Steptobacillus moniliformis

SPS = sodium polyanthenol sulfonate