sequencing, precision medicine

what is a contig

consensus sequence partially reconstructed sequence

what makes a quality/trustworhty sequence assembly?

high fold coverage - 50Cx or more

why would dna sequencing fail

error with extraction

With medication development in what order do we do the three major stages and why

culture study, animal research, human trials - make sure its safe/ no bad side effects

what is precision medicine

using a persons DNA sequnce to see if drug will work for them

what is microarray and how does it measure protein

analyzes protein expression on a large scale by detecting presence and quantity of specific molecules

what is the structure/ parts of an amino acid

R gorup, Amine group, carboxyl group, Hydrogen, conneteed to central/ a carbon

what bond connects amino acid together and what does it prodcue

peptide bond N of amine - C of carboxyl - makes H2O

what makes something hydrophobic

non polar

what make something hydrophilic

polar

what does aliphatic mean

organic compound with open chain structure

what does amphipathic mean

molecule has both hydrophobic and hydrophilic regions

what is an alpha helix and beta sheet

A - coils B - forms a sheet

what is the function of a chaperonin protein

assists in folding of proteins, puts in lowest energy state

what is a disulfide bond and wha tis their purpose in protien folding

2 S, put secondary structures together

what amino acid forms disulfide bonds

cytosine

what is a primary protien structure

amino acid chain

what is a secondary protien structure

folding of polypeptide chain into a helix or B sheet

what is a tertiary protien structure

1 complete protein

what is a quatrenary protien structure

2 or more proteins together

what is a domain

independently folidng distinct structual and funciton unit within a protein

what is a subunit

individual polypeptide chain that assembles with other chains to form a larger protein complex

why is the rotation of R-groups important in protien folding

flexibility, interactions, stability, function

What si the difference between a parallel and antiparallel beta sheet

p - same direciton ap - oposite direction , H bodnings, more common/ stable

put yes

yes

what is this called

helix turn helix

what is this called

four helix bundle

what is this called

coiled coil

what is this called

B hairpin

what is this called

antiparallel B sheet

what is this called

B barrel

what is this called

B a B motif

what is this called

a/B barrel domain

what is this called

greek key motif

what determines tertiary structure

domain and domain orientation

what is this showing, what does it represent

van der waals surface - forces

what is this showing, what does it represent

sticks - bonds of atoms

what is this showing, what does it represent

a - carbon trace - carbons

what is this showing, what does it represent

ribon diagram - a helix vs. b sheets

what is this showing, what does it represent

electrostatic surface representation basic vs. acid, + vs -