Enzyme Kinetics and Cofactors - Page 1 Notes

Five QA-style flashcards based on Page 1 notes.

What happens to the rate of an enzyme-catalyzed reaction as substrate concentration increases before saturation?

The rate increases with substrate concentration as more active sites are occupied, until saturation is reached and the rate plateaus at Vmax.

What does saturation kinetics describe in enzyme reactions?

A condition in which increasing substrate concentration no longer increases the rate because all enzyme active sites are occupied, resulting in Vmax.

What are cofactors in enzymology?

Nonprotein entities that must bind to a particular enzyme before the reaction can proceed.

What is a coenzyme?

An organic cofactor that binds to the enzyme as a second substrate and is essential for achieving maximal enzymatic activity.

Name two examples of cofactors mentioned in the notes.

NAD (nicotinamide adenine dinucleotide) and NADP (nicotinamide adenine dinucleotide phosphate).