APOLLON: Mycology

FUNGI

members of plant kingdom, lacks roots and stems, lacks chlorophyll

ASEPTATE HYPHAE

coenocytic hypha

SUPERFICIAL MYCOSES

mycoses that produce no cellular response by host

ECTOTHRIX

arthroconidia on outside of hair as well as hyphae within hair shaft

BLASTOCONIDIA

true yeasts prod this in asexual stage

HAVE DIVISIONS

septate hyphae

SPORANGIOPHORE

molds with aseptate hyphae (zygomycetes) prod specialized hyphal struc

MYCELIUM

basic, intertwining struc of molds

DIRECTLY FROM THE HYPHAE BY FRAGMENTATION

arthroconidia are formed

VEGETATIVE HYPHAE

mycelia within the colony that grow into the substrate

INTERCALARY

chlamydospores that grow within the hyphae

BLASTOSPORES

budding forms in which the daughter cells forms as a bud from a single mother cell or forms laterally from a mycelium or pseudomycelium

ASEXUAL SPORES

conidia

MULTICELLULAR

macroconidia

VESICLE

swollen portion of conidiophore

EXHIBIT THERMAL DIMORPHISM

statement that does NOT apply to true yeasts

ECHINULATE

conidia with rough and spiny surface

CATENATE

conidia in chains

INCREASE THE RATE OF CLEARING

slide gently heated in prep temp mounts of fungal elements

10% KOH

clearing agent to eliminate debris and make fungal elements more prom in skin, hair, nails

COTTON BLUE

stain in aman med

INDIA INK STAIN

negative stain

RED TO VIOLET

glycogen in PAS

CALCOFLUOR WHITE

used for sharp delineation of fungal elements by fluorescent microscopy

PAS

restain H&E slides to visualize fungal wall

FLUORESCE A GREEN COLOR

fungal elements in acridine orange

CONTAIN CHLORAMPHENICOL AND CYCLOHEXIMIDE

mycosel differ from SDA

SABORAUD'S DEXTROSE AGAR

primary isolation and maintenance of fungal cultures

PHENOL RED

indication in dermatophyte test med

PIGMENT PRODUCTION

cornmeal agar with 1% glucose diff T. rubrum vs T. mentagrophyte

INCUBATED AT 25-30C

fungal culture

30 DAYS

fungal culture to be held before reporting as negative

CYCLOHEXIMIDE AND CHLORAMPHENICOL

antibiotics added to media for isolation of pathogenic fungi from contam material

CORNMEAL AGAR

media that stim prod of chlamydo spores

CZAPEK'S AGAR

medium for aspergillus

V-SHAPED PENETRATION OF HAIRSHAFT

positive result in hair baiting test

RINGWORM

tinea infec

TRICHOSPORON BEIGELLI

white piedra

RINGWORM OF NAILS (NAIL PLATE)

tinea unguium

TRICHOPHYTON SCHOENLEINII

favus type of tinea capitis

MICROSPORUM AUDOUINII

anthrophilic

MICROSPORUM CANIS

zoophilic

MICROSPORUM GYPSEUM

geophilic

MICROSPORUM CANIS

dermatophyte fluoresce under Wood's lamp

DERMATOPHYTE

colonization by dermatophytic fungi of hair skin and nails

MICROSPORUM, EPIDERMOPHYTON, TRICHOPHYTON

causes of dermatophytes (MET)

EPIDERMOPHYTON FLOCCOSUM

macroconidia that are large multicellular and club shaped with smooth walls

T. TONSURANS

balloon forms

T. MENTAGROPHYTES

urease (+)

hair baiting (+)

T. RUBRUM

urease (-)

hair baiting (-)

TRICHOPHYTON SCHOENLEINII

favic chandeliers

SCLEROTIC BODIES

diagnosis for chromoblastomycosis

CIGAR SHAPED YEAST CELLS

yeast phase of S. schenckii on culture med at 37C

ASTEROID BODY

result of ab-ag rxn in cases of sporotrichosis

SLEEVE

older culture os Sporothrix schenckii, single conidia borne along sides of hyphae

PHAEOHYPHOMYCOSIS

infec caused by dematiaceous saprobes that invade organs of immunosuppressed hosts

PSEUDOALLESCJERIA BOYDII

most common cause of mycetoma

SURFACE AND REVERSE SIDE DARK

dematiaceous fungi

PHIALOPHORA AND CLADOSPORIUM

example of dematiaceous fungi

CLADOSPORIUM (CHROMOBLASTOMYCOSIS)

sclerotic bodies

EXCEPT EXOPHIALA

chromoblastomycosis: fonsecaea, cladosporium, phialophora

MONOMORPHIC

mold at 35-37C and at 25C

EUMYCOTIC

true fungi that prod hyphae and spores

ASPERGILLUS, PENICILLIUM MARNEFFEI AND FUSARIUM MONILIFORME

infec by hyaline saprobes that invade most tx or body fd of immunosuppressed hosts

PSEUDOALLESCHERIA BOYDII

cleistothecia containing ascosporea

FUNGUS LIKE BACTERIA

actinomyces

ACTINOMYCES ISRAELII

anaerobic, gram (+) but not acid fast and does not stain with fungal stains

CRYPTOCOCCUS NEOFORMANS

characterized with a capsule

CYCLOHEXIMIDE

culturing cryptococcus on SDA, this antibiotic should not be in med

CRYPTOCOCCUS

mistaken for lymphycte in CSF

BLASTOMYCES

mistaken for myelin globules in sputum

ASSIMILATES CREATININE

cryptococcus neoformans prod brown color on thistle extract (birdseed or staib agar)

UREASE (+), INOSITOL (+), NITRATE (-)

cryptococcus neoformans

ANTIGEN

c. neoformans latex aggn test on csf detects

BLASTOSPORES, CHLAMYDOSPORES, PSEUDOHYPHAE

candida albicans prod

THRUSH

another name for oral candidiasis

GERM TUBE

c. albicans prod which struc in serum

CHLAMYDOSPORE

cornmeal agar plus tween 80 is used to id c. albicans through org prod of

RAPID YEATSLIKE GROWTH

geotrichum candidum colonies appear

YEAST FORM (TISSUE)

lab workers are in no danger when handling this form of dimorphic fungi

MONOMORPHIC MOLD

sepedonium prod spiny tuberculate macroconidia

RECTANGULAR EVENLY STAINING ARTHROCONIDIA

prod by g. candidum

YEAST CELLS WITHIN MACROPHAGE

tx phase h. capsulatum

TUBERCULATE CHLAMYDOSPORES, TUBERCULATE MACROCONIDIA

mycelial phase of h. capsulatum

LEISHMANIA HAS CENTRAL NUCLEAR MATERIAL

leishmania can be diff from histoplasma

EXOANTIGEN TEST

test that may be used instead of conversion when id dimorphic fungi

CONIDIOPHORES THAT RESEMBLE LOLLIPOPS

mycelial form of b. dermatitidis

BLASTOMYCES

yeast form with single broad based bud

SMALL PEAR SHAPED CONIDIA CALLED LOLLIPOPS

mycelial form of blastomycosis

PARACOCCIDIOIDES BRASILIENSIS

yeastlike forms with multiple buds

NEVER BUD

immature spherules of Coccidioides are diff from yeast form of Blastomyces

COCCIDIOIDES IMMITIS

major biohazard to lab personnel

MICROSCOPIC IMMUNODIFFISION TEST

exoag test

H AND/OR M BANDS

exoag test for h. capsulatum

LEISHMANIA WILL NOT STAIN

diff h. capuslatum from leishmania using fungus stain

A BAND

exoag test for b. dermatitidis

COCCIDIOIDES IMMITIS

spherule filled with endospores

MUCOR AND RHIZOPUS

zygomycosis

BROAD AND NONSEPTATE

fungal elements in zygomycosis