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What does oxidation and reduction mean?
OIL- oxidation is loosing electrons
RIG- reduction is gaining electrons
How do electrons move in biology?
with a hydrogen atom
Glucose becomes ____ to form CO2 in cellular respiration
oxidized
O2 becomes ____ to form water in cellular respiration
reduced
What are the reactants and products of cellular respiration?
C6H12O6 + O2 -> CO2 + ATP+ H2O
What electron carriers pick up electrons in glycolysis? What electron carriers pick up electrons in the Krebs cycle?
NADH (glycolysis), FADH and NADH (krebs)
What is one thing that differentiates glycolysis from the Krebs and Electron Transport Chain? what does it not require?
oxygen
What 3 steps of cellular respiration occur in the mitochondria?
oxidation of pyruvate, krebs cycle, electron transport cain
What does a catabolic pathway do? what about an anabolic pathway?
catabolic= break down
anabolic= build molecules
Which step of cell respiration occurs int eh cytosol?
glycolysis
Where does the Krebs Cycle occur?
mitochondrial matrix
What is the net gain of ATP in glycolysis?
2 ATP
How many ATP's are invested in glycolysis?
2 ATP
How many total ATPs are generated in glycolysis? (not net gain)
4 ATP
In cellular respiration, glucose is split into what?
2 pyruvates
1 glucose molecule will produce how many turns of the Krebs Cycle? why?
2 turns in the cycle because there are 2 pyruvates
what molecule exits glycolysis and need to be oxidized in order to enter the next cycle?
pyruvate
When carbon is is lost in the ______ cycle, it is lost in this form of ________
krebs
-CO2
What occurs in the inner mitochondrial membrane?
electron transport chain of cellular respiration
How is ATP synthase powered in this picture?
hydrogn ions accumulate in teh intermembrane space, which then diffuse through ATP synthase to the mitochondrial matrix to create ATP
-photos in group chat
What is being shown here?
lactic acid fermentation
-photos in group chat
Which type of fermentation does yeast carry out?
alcoholic fermentation
Which type of fermentation results in the production of CO2?
alcoholic fermentation
________ is the factory of producing energy and sugar in photosynthesis
chloroplast
Where is the chlorophyll (and other pigments) located in chloroplast?
thylakoid memebrane
What is produced in the light dependent reactions of photosynthesis? 3 things
O2, ATP, NADPH
Light independent reactions of photosynthesis are also known as what?
Calvin Cycle
What does the Calvin Cycle produce?
NADP+, ADP, and SUGAR
What is the first protein in the light reactions of photosynthesis?
photosystem II
Where do hydrogen ions accumulate in the light reactions of photosynthesis?
thylakoid space
where do the electrons come from in the light reactions of photosynthesis? ________ energizes these electrons to travel down the ___________
Light
-H20 energizes these electrons to travel down the -Electron transport chain
What is the reduced form of photosynthesis electron carrier?
NADPH
What is the purpose of photosystem I?
to re-energize electrons traveling through the electron transport chain
Where does the Calvin Cycle occur?
stroma
What is the waste product of photosynthesis? Why is this good for us?
O2, because O2 is needed for cellular respiration as a reactant
What are the reactants and products of photosynthesis?
CO2 + H2O + light energy -> glucose + O2
What enzyme is responsible for fixing carbon in photosynthesis?
RuBisCo
RuBisco combines from ________ with __________
-CO2
-RuBP
What 3 carbon sugar is created in the Calvin Cycle?
G3P
ATP made during glycolysis is made via what type of phosphorylation?
substrate level phophorylation
What is the factors of oxygen in the electron transport chain of cellular respiration?
final electron acceptor of electron transport
In addition to ATP, what are the end products of glycolysis?
NADH and pyruvate
Carbon dioxide is released during which of the following stages of cellular respiration? (2 steps)
oxidation of pyruvate to acetyl CoA and the Krebs
What enters the Krebs cycle?
Acetyl-CoA
What is the another name for Krebs Cycle? Why is it called this?
citric acid because citrate (citric acid) is the first intermediate formed in the cycle
What is the total energy yield for cellular respiration?Where is most of this energy formed?
36-38 total ATP
-most formed in the electron transport chain
What would happen if you were growing a plant and placed it under a green light? Explain
it wouldn't grow well because pigments in the plants do not absorb well for green wavelenghts
Where does O2 come from in photosynthesis? What molecule?
splitting of water
What lab did we use KOH and what was the purpose?
KOH was used in the CELLULAR RESPIRATION LAB to absorb CO2 in the respirometers
-this allowed us to measure O2 consumption of peas
What lab did we use DPIP and what was its function? What molecules did it act as?
Photosynthesis lab- to act as an electron carrier (NADP+)
DPIP(blue) would turn to DPIPH (colorless) in the presence of chloroplasts, indicating light dependent reaction is occurring. This color change was measurable with the spec-20 machine.
Fermentation
a catabolic process that makes a limited amount of ATP from glucose or other organic molecules without an electron transport chain and that produces a characteristic end product, such as ethyl alcohol or lactic acid
Aerobic respiration
a catabolic pathway for organic molecules, using oxygen as the final electron acceptor in an electron transport chain and ultimately producing ATP. This is the most efficient catabolic pathway and is carried out in the most eukaryotic cells and many prokaryotic organisms
cellular respiration
the catabolic pathways of aerobic and anaerobic respiration, which break down organic molecules and use an electron transport chain for the production of ATP
Redox reaction
- a chemical reaction involving the complete or partial transfer of one or more electrons from one reactant to another; short for reduction oxidation reaction
oxidation
the complete or partial loss of electrons from a substance involved in a redox reaction
Reduction
the complete or partial addition of electrons to a substance involved in a redox reaction
reducing agent
the electron donor in a redox reaction
oxidizing agent
the electron acceptor in a redox reaction
NAD+
Nicotinamide adenine dinucleotide, a coenzyme that cycles easily between oxidized (NAD+) and reduced (NADH) states, thus acting as an electron carried
Electron Transport Chain
a sequence of electron carrier molecules (membrane proteins) that shuttle electrons down a series of redox reactions that release energy used to make ATP
Glycolysis
a series of reactions that ultimately splits glucose into pyruvate. Glycolysis occurs in almost all living cells, serving as the starting point for fermentation or cellular respiration
citric acid cycle
a chemical cycle involving eight steps that completes the metabolic breakdown of glucose molecules begun in glycolysis by oxidizing acetyl CoA (derived from pyruvate) to carbon dioxide; occurs within the mitochondrion in eukaryotic cells and in the cytosol of prokaryotes; together with pyruvate oxidation, the second major stage in cellular respiration
oxidative phosphorylation
the production of ATP using energy derived from the redox reactions of an electron transport chain; the third major sage of cellular respiration
Substrate-level phosphorylation
the enzyme-catalyzed information of ATP by direct transfer of a phosphate group to ADP from an intermediate substrate in catabolism
ATP synthase
a complex of several membrane proteins that functions in chemiosmosis with adjacent electron transport chains, using the energy of a hydrogen ion (proton) concentration gradient to make ATP. ATP synthase are found in the inner mitochondrial membranes of eukaryotic cells and in the plasma membranes of prokaryotes
Chemiosmosis
an energy coupling mechanism that uses energy stored in the form of a hydrogen ion gradient across a membrane to drive cellular work, such as the synthesis of ATP. Under aerobic conditions, most ATP synthesis in cells occurs by chemiosmosis
Cytochromes
an iron-containing protein that is a component of electron transport chains in the mitochondria and chloroplasts of eukaryotic cells and the plasma membranes of prokaryotic cells
Proton-motive force
the potential energy stored in the form of a proton electrochemical gradient, generated by the pumping of hydrogen ions (H+) across a biological membrane during chemiosmosis
Alcohol Fermentation
glycolysis followed by the reduction of pyruvate to ethyl alcohol, regenerating NAD+ and releasing carbon dioxide
Lactic Acid Fermentation
glycolysis followed by the reduction of pyruvate to lactate, regenerating NAD+ with no release of carbon dioxide
Obligate Anaerobes
an organism that only carries out fermentation or anaerobic respiration. Such organisms cannot use oxygen and in fact may be poisoned by it
facultative anaerobes
an organism that makes ATP by aerobic respiration if oxygen is present but that switches to anaerobic respiration or fermentation if oxygen is not present.
beta oxidation
a metabolic sequence that breaks fatty acids down to two-carbon fragments that enter the citric acid cycle as acetyl CoA
Photosynthesis
the conversion of light energy to chemical energy that is stored in sugars or other organic compounds; occurs in plants, algae, and certain prokaryotes
autotrophs
an organism that obtains organic food molecules without eating other organisms or substances derived from other organisms. Autotrophs use energy from the sun or from oxidation of inorganic substances to make organic molecules form inorganic ones
heterotrophs
an organism that obtains organic food molecules by eating other organisms or substances derived from them
Mesophyll
leaf cells specialized for photosynthesis. In C3 and CAM plants, mesophyll cells are located between the upper and lower epidermis; in C4 plants, they are located between the bundle-sheath cells and the epidermis
Stomata
a microscopic pore surrounded by guard cell sin the epidermis of leaves and stems that allows gas exchange between the environment and the interior of the plant
Stroma
the dense fluid liquid within the chloroplast surrounding the thylakoid membrane and containing ribosomes and DNA; involved in the synthesis of organic molecules from carbon dioxide and water
Thylakoids
a flattened, membranous sac inside a chloroplast. Thylakoids often exist in stacks called grana that are interconnected; their membranes contain molecular "machinery" used to convert light energy to chemical energy
Granum aka Grana
a stack of membrane-bounded thylakoids in the chloroplast. Function in the light reactions of photosynthesis
Clorophyll
- a green pigment located in membranes within the chloroplasts of plants and algae and in the membranes of certain prokaryotes. Chlorophyll a participates directly in the light reactions, which convert solar energy to chemical energy
Light Reactions
the first of two major stages in photosynthesis (before the Calvin cycle). These reactions, which occur on the thylakoid membranes of the chloroplast or on membranes of certain prokaryotes, convert solar energy to the chemical energy of ATP and NADPH, releasing oxygen in the process
Calvin Cycle
the second of two major stages in photosynthesis (following the light reactions), involving fixation of atmospheric CO2 and reduction of the fixed carbon into carbohydrate
NADP+
nicotinamide adenine dinucleotide phosphate, an elector acceptor that, as NADPH, temporarily stores energized electrons produced during the light reactions
Carbon Fixation
the initial incorporation of carbon from CO2 into an organic compound by an autotrophic organism (a plant, another photosynthetic organism, or a chemoautotrophic prokaryote)
wavelength
the distance between crests of waves, such as those of the electromagnetic spectrum
electromagnetic spectrum
the entire spectrum of electromagnetic radiation, ranging in wavelength from less than a nanometer to more than a kilometer
Visible Light
the portion of the electromagnetic spectrum that can be detected as various colors by the human eye, ranging in wavelength form about 380nm to about 750nm
Photons
a quantum, or discrete quantity, of light energy that behaves as if it were a particle
Spectrophotometer
an instrument that measures the proportions of light of different wavelengths absorbed and transmitted by a pigment solution
Absorption Spectrum
the range of a pigment's ability to absorb various wavelengths of light; also a graph of such a range
Chlorophyll a
a photosynthetic pigment that participates directly in the light reactions, which convert solar energy to chemical energy
Chlorophyll b
an accessory photosynthetic pigment that transfers energy to chlorophyll a
Action spectrum
a graph that profiles the relative effectiveness of different wavelengths of radiation in driving a particular process
Carotenoids
an accessory pigment, either yellow or orange, in the chloroplasts of plants and in some prokaryotes. By absorbing wavelengths of light that chlorophyll cannot, carotenoids broaden the spectrum of colors that can drive photosynthesis
Photosystem
a light-capturing unit located in the thylakoid membrane of the chloroplast or in the membrane of some prokaryotes, consisting of a reaction-center complex surrounded by numerous light-harvesting complexes. There are two types of photosystems, I and II; they absorb light best at different wavelengths
Reaction-Center Complex
a complex of proteins associated with a special pair of chlorophyll a molecules and a primary electron acceptor. Located centrally in a photosystem, this complex triggers the light reaction of photosynthesis, excited by light energy, the pair of chlorophylls donates an electron to the primary electron acceptor, which passes an electron to an electron transport chain
Light-harvesting complex
a complex of proteins associated with pigment molecules (including chlorophyll a, chlorophyll b, and carotenoids) that captures light energy and transfers it to a reaction-center pigments in a photosystem
Primary Electron Acceptor
in the thylakoid membrane of a chloroplast or in the membrane of some prokaryotes, a specialized molecule that shares the reaction-center complex with a pair of chlorophyll a molecules and that accepts an electron from them