Gram-Negative Rods (Enterobacteriaceae)

all enterobacteriaceae are catalase ____.

positive

all enterobacteriaceae are oxidase ____.

negative

SS: no lactose fermenter/no H2S (colorless colonies)

MAC: no lactose fermentation (colorless colonies)

HEA: no sugar fermentation/no H2S (remains as blue-green colonies)

shigella sonnei; morganella morganii

SS: no lactose fermenter/H2S production (colorless colonies with black)

MAC: no lactose fermentation (colorless colonies)

HEA: no sugar fermentation/H2S production (remains as blue-green with black center colonies)

salmonella typhimurium; proteus mirabilis; citrobacter freundii

SS: no lactose fermenter/no H2S (colorless colonies)

MAC: no lactose fermentation (colorless colonies)

HEA: sugar fermentation/no H2S (yellow colonies)

yersinia enterocolitica

SS: lactose fermenter/no H2S (pink colonies)

MAC: lactose fermentation (pink colonies)

HEA: sugar fermentation/no H2S (yellow colonies)

escherichia coli; klebsiella pneumoniae; enterobacter cloacae

KIA: K/A (red slant/yellow butt)

LIA: K/A (purple slant/yellow butt)

Indole: negative

shigella sonnei

KIA: K/A (red slant/yellow butt) with H2S production

LIA: K/K (purple slant/purple butt)

Indole: negative

salmonella typhimurium

KIA: K/A (red slant/yellow butt)

LIA: K/A (purple slant/yellow butt)

Indole: positive

yersinia enterocolitica

KIA: K/A (red slant/yellow butt) with H2S production

LIA: K/A (purple slant/yellow butt)

Indole: negative

citrobacter freundii

KIA: A/A (yellow slant/yellow butt)

LIA: K/K (purple slant/purple butt)

Indole: positive

escherichia coli

KIA: A/A (yellow slant/yellow butt)

LIA: K/K (purple slant/purple butt)

Indole: negative

klebsiella pneumoniae

KIA: K/A (red slant/yellow butt)

LIA: R/A (red slant/yellow butt)

Indole: positive

morganella morganii

KIA: K/A (red slant/yellow butt) with H2S production

LIA: R/A (red slant/yellow butt)

Indole: negative

proteus mirabilis

KIA: A/A (yellow slant/yellow butt)

LIA: K/A (purple slant/yellow butt)

Indole: negative

enterobacter cloacae

list the bacteria that may display K/A instead of A/A for the KIA slant.

klebsiella pneumoniae; enterobacter cloacae