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What is the goal of electrophoresis?
To separate DNA (STRs) by size due to negative charge.
What dictates the number of samples you can run in gel electrophoresis?
The size of the gel and the number of wells.
Between agarose and PAGE gel, which resolves smaller DNA molecules?
PAGE
What are the limitations of PAGE gel?
Time consuming
Not easily automated
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What is the “native” condition of DNA in gel electrophoresis?
The double-stranded or non-denatured form.
What chemicals can be added to the DNA sample in gel electrophoresis to prevent the formation of hydrogen bonds between complementary ssDNA molecules?
Formamide or urea.
What are the general steps of capillary electrophoresis (CE)?
Sample injection
Separation
Detection of STR alleles
