Lab Specimen

Used in all exercises (denoted by numbers) and their collective properties as tested in lab

Eschericia coli

2) Morphology: creamy white, translucent, smooth, circular, convex

Oxygen tolerance: facultative anaerobe

3) Cellular morphology: single bacillus or diplobacilli (medium)

4) Gram-stain: negative (pink)

13) Culture growth:

MAC → pink; lactose-fermenting

BAP → no change; gamma hemolysis

Bacillus subtilis

2) Morphology: white, dull, dry, irregular, flat/raised

Oxygen tolerance: obligate aerobe

3) Cellular morphology: streptobacilli (largest)

4) Gram stain: positive (purple)

Serratia marcescens

2) Morphology: red, opaque, smooth, circular, convex

Oxygen tolerance: facultative anaerobe

Proteus Vulgaris

3) Motile? Yes

4) Cellular morphology: single bacillus

Gram stain: negative (pink)

Staphylococcus epidermidis

8)Morphology: creamy white, opaque, shiny, circular, raised

3) Cellular morphology: staphylococci (smallest)

Motile? No

4) Gram-stain: positive (purple)

13) Culture growth:

MSA → red; non-mannitol fermenting

BAP → no change; gamma hemolysis

Enterococcus faecalis

Cell morphology: diplo/streptococci

4) Gram-stain: positive (purple)

Saccharomyces cerevisiae

Yeast

Cell morphology: cocci but also can be bacilli

4) Gram-stain: positive (purple)

Mycobacterium smegmatis

5) Acid-fast stain: positive (red)

Staphylococcus aureus

Morphology: yellow, opaque, glistening, circular, convex colonies

Cellular morphology: staphylococci

Gram-stain: positive (purple)

13) Culture growths:

MSA → yellow; mannitol fermentation

BAP → yellow; beta-hemolysis

Pseudomonas aeruginosa

Morphology: blue-green, opaque, crust-like, irregular, umbonate

Cell morphology: single or diplo/streptobacilli

Gram-stain: negative (pink)

13) Culture growths:

MAC → colorless; non-lactose-fermenting gram-negative

BAP → yellow; beta hemolysis