bio 305 exam 3

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Sterilization

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Completely kills or removes all forms of microbial life.

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Disinfection

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Eliminates most pathogens, but not all.

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52 Terms

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Sterilization

Completely kills or removes all forms of microbial life.

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Disinfection

Eliminates most pathogens, but not all.

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Sanitization

Reduces microbial numbers to safe levels.

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Antisepsis

Destroying pathogens on living tissue.

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Degerming

Physically removing microbes from a surface (e.g., handwashing).

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Bacteriostatic

Inhibits the growth of bacteria.

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Bactericidal

Kills bacteria.

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Autoclave

Uses steam under pressure to sterilize equipment and materials.

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Pasteurization

Heat treatment that reduces spoilage organisms and pathogens in food.

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Genotype

An organism’s genetic makeup.

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Phenotype

The observable characteristics or traits of an organism.

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Mutation

A change in the DNA sequence.

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Mutagen

A physical or chemical agent that causes mutations.

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Horizontal gene transfer

The movement of genetic material between bacteria.

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Transformation

Uptake of naked DNA from the environment by a bacterial cell.

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Transduction

Transfer of DNA from one bacterium to another via a bacteriophage.

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Conjugation

Transfer of genetic material between bacteria using a pilus.

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DNA replication

The process of making an identical copy of DNA.

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Transcription

Process of copying DNA into RNA.

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Translation

Process of converting RNA into a protein.

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mRNA

Messenger RNA; carries genetic code from DNA to ribosome.

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tRNA

Transfer RNA; brings amino acids to the ribosome.

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rRNA

Ribosomal RNA; forms part of the ribosome.

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Codon

A sequence of three bases in mRNA that codes for an amino acid.

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Anticodon

A three-base sequence in tRNA complementary to a codon in mRNA.

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Enzyme

A protein that speeds up chemical reactions.

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Substrate

The molecule upon which an enzyme acts.

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Active site

The part of an enzyme where the substrate binds.

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Catabolism

Breakdown of molecules to release energy.

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Anabolism

Synthesis of complex molecules, requiring energy.

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ATP

Adenosine triphosphate; the main energy currency of the cell.

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Binary fission

A form of asexual reproduction in bacteria.

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Lag phase

The adjustment period where bacteria prepare to divide.

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Log phase

The phase of rapid bacterial growth and division.

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Stationary phase

Phase where growth rate equals death rate due to limited resources.

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Death phase

Phase where bacterial cells die off due to nutrient depletion.

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Capsule

Sticky, protective layer outside the cell wall; helps evade immune response.

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Flagella

Tail-like structures used for bacterial movement.

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Pili

Hair-like structures used for attachment or DNA transfer.

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Endospores

Dormant, tough, non-reproductive structures for bacterial survival in harsh conditions.

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Pathogen

A microorganism that causes disease.

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Antigen

A substance that triggers an immune response.

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Antibody

A protein produced by B cells that binds to antigens.

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Innate immunity

Non-specific, first-line defense mechanisms (e.g., skin, fever).

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Adaptive immunity

Specific immune response with memory, involving B and T lymphocytes.

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