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What are the methods to detect positive selection?
Ka/Ks test
McDonald-Kreitman (MK) test
Fixation index (Est)
Linkage disequilibrium (LD)
What is the Ka/Ks test suitable for?
Suitable between species (Ka = non-synonymous, Ks = synonymous substitutions)
What is the McDonald-Kreitman (MK) test for?
For comparison between species and within species
What is the fixation index (Est) for?
Testing different in allele frequency between populations
What is linkage disequilibrium (LD) for?
Look for non-random association of alleles at loci
What are molecular clocks?
Changes in homologous proteins between species is directly proportional to time