Protein-Ligand and Protein-Protein Interactions

Protein-Ligand Interaction

Binding of a protein to a specific ligand, defined by tertiary and quaternary structures

Recognition Domains

Protein regions responsible for specific interactions with ligands

Substrate Specificity

Antibodies binding to specific antigens based on Ig fold sequences

Ka

Association rate constant measuring antibody-antigen binding affinity

ELISA

Enzyme-linked immunosorbent assay for detecting antibody-antigen interactions

Scatchard Plots

Graphical method to determine antibody binding constants from saturation data

Affinity vs Avidity

Affinity measures how well an antibody binds, avidity quantifies multiple binding ways

Protein-Protein Interactions

Quantitative assessment of how tightly proteins interact

Yeast Two Hybrid (Y2H)

Screening method detecting transient protein interactions with β-gal activity read-out

Co-immunoprecipitation (Co-IP)

Technique confirming protein complex formation using antigen-antibody interactions

Isothermal Calorimetry (ITC)

Method measuring heat exchange in binding events to determine interaction affinity

Surface Plasmon Resonance (SPR)

Technique measuring binding affinities without requiring large material amounts

Tag Pull-Down

Method to confirm protein complex formation using tagged proteins and affinity resin

Profilin vs Thymosin vs Cofilin

Distinct proteins regulating actin dynamics and filament formation

Molecular Motors

Proteins facilitating intracellular transport along actin filaments

Myosin-Actin Filaments

Structures involved in muscle contraction powered by myosin motors