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State the function of the mitochondria.
The site of respiration in plant and animal cells
State the function of the chloroplasts.
The site of photosynthesis in a plant cell
State the function of the cell membrane.
Controls what comes in and goes out of all cells
State the function of the cytoplasm.
Site of chemical reactions in the cell
State the function of the vacuole.
A large, cell sap-filled space in plant cells
State the function of the nucleus.
Contains DNA and controls the cell activities
State the function of the ribosomes.
Bead-shaped organelles which are the site of protein synthesis
Describe the composition and functions of the cell wall.
Are fully permeable, give the cell support and shape, and are made of cellulose (plants) and different materials in bacteria and fungi
What is the name of the small circular pieces of DNA in bacterial cells?
plasmids
Where is the genetic material of a bacterial cell found?
Free in the cytoplasm
State three differences between animal and plant cells.
Plant cells have chloroplasts, vacuole and cellulose cell wall. Animal cells do not
Convert 5 mm to µm.
5000 µm
Name the two substances that the cell membrane consists of
phospholipids and proteins
What does a selectively permeable membrane mean?
Membrane that only allows some types of substances to pass through
Define passive transport
Down the concentration gradient and does not require energy.
Name two types of passive transport in cells
Diffusion and osmosis
Define diffusion
The movement of molecules down a concentration gradient from a higher to a lower concentration
Define osmosis
The movement of water molecules from a high water concentration to a low water concentration through a selectively permeable membrane
Name a substance that diffuses into our cells for use.
Oxygen, glucose
Name a substance that diffuses out of our cells to be removed.
Carbon dioxide, urea
What does 'net movement' mean?
Overall movement: the direction that most of the particles are moving in
State three factors that affect the rate of diffusion.
Concentration gradient, temperature, surface area
How does concentration difference affect the rate of diffusion?
Higher concentration difference/Steeper concentration gradient --> faster diffusion
If a cell is in a solution of a lower water concentration, water will ___ (enter/leave) the cell.
Leave
If a cell is in a solution of a higher water concentration, water will ___ (enter/leave) the cell.
Enter
What will happen to an animal cell in a lower water concentration?
Water leaves cell --> shrinks
What will happen to an animal cell in a higher water concentration?
Water enters cell --> burst
Why do animal cells burst in lower water concentration solutions?
No cell wall
What will happen to a plant cell in a lower water concentration?
Water leaves cell --> plasmolysed
What will happen to a plant cell in a higher water concentration?
Water enters cell --> turgid
What does 'plasmolysed' mean?
The cell membrane pulls away from cell wall
How do we calculate % change in mass?
(final mass - initial mass)/initial mass x 100
Define active transport
The movement of molecules and ions against the concentration gradient using energy and membrane proteins
Explain the importance of active transport in plants.
Root hair cells carry out active transport to absorb mineral ions effectively in dilute soil
State one adaptation cells may have if they need to carry out active transport.
Lots of mitochondria to release energy via aerobic respiration
How does active transport differ from diffusion and osmosis?
Active transport uses energy, diffusion and osmosis do not
State two differences between diffusion and osmosis.
D: Any particles, does not need a membrane; O: Water specific, needs partially permeable membrane
What is the function of DNA
The genetic information for making proteins
Describe the structure of a DNA molecule
A double-stranded helix
Name the three parts of a DNA molecule.
A phosphate group, a sugar molecule and a nucleotide base
Name the complementary base pairs
A-T, C-G
The base sequence determines
The amino acid sequence in a protein
Describe mRNA
A complementary copy of the code from the DNA, in the nucleus, carried to a ribosome
Which organelle synthesises proteins?
ribosome
What is a gene?
a section of DNA which codes for a protein
What are chromosomes?
Structures in the nucleus that carry genes, made of DNA
Name the structure within which DNA is contained.
Chromosomes
What does the sequence of amino acids control?
Controls the shape and function of proteins
What are the 5 types of proteins?
Structural, hormones, antibodies, receptors, enzymes
Describe the role of structural proteins
Hold tissues together e.g. collagen
Define hormones
Chemical messengers which carry a message from a gland to a receptor via the blood
What are antibodies?
Proteins, made by white blood cells, which destroy pathogens by binding to them
Define receptors
Bind to a hormone and tell the target cell what to do.
Define enzymes
Biological catalysts
Describe a biological catalyst
Speeds up chemical reactions in cells and remain unchanged in the process
Define active site
Where the substrate fits into the enzyme and is a complementary shape to it
Define substrate
The reactant(s) in a reaction, and is specific to an enzyme
Define product
The molecule(s) made by an enzyme-controlled reaction
Define optimum conditions
The temperature and pH which the enzymes are most active
Define denatured
When the active site changes shape, the substrate no longer fits, and this affects the rate of the reaction
An example of a degradation reaction is
Starch then amylase then maltose
An example of a synthesis reaction is
G-1-P phosphorylase starch
How does temperature affect enzymes' rate of reaction?
As temperature increases ,the rate of reaction increases until after the optimum is reached when rate of reaction rapidly decreases to zero
Why do enzymes stop working past their optimum temperature?
Enzyme is denatured (substrate can no longer bind to active site)
Why do enzymes not work well at lower temperatures?
There is not enough kinetic energy for the substrate to collide and bind to active site
How does a change in pH affect enzyme structure?
pH change affects the bonds holding enzyme structure together, causing it to denature
What is genetic engineering?
Transferring genetic information (DNA) from one species' cell into another
List the 6 steps in genetic engineering, in order:
1. Identify the gene required on the source chromosome 2. Extract (cut out) the required gene 3. Extract the plasmid from the bacterial cell 4. Insert the required gene into a bacterial plasmid 5. Insert the plasmid into the host cell (bacteria) 6. to produce a GM organism
What 2 things are enzymes are needed for in genetic engineering
Cut the DNA to extract the gene, cut the plasmid open and seal the gene into the plasmid
State the word equation for aerobic respiration.
Glucose + Oxygen --> Carbon dioxide + Water
Respiration is defined as
A series of enzyme-controlled reactions which release the chemical energy stored in glucose
What is the energy released from respiration is transferred to?
ATP
State 5 uses for the energy transferred from ATP in the cell
muscle cell contraction, cell division, protein synthesis, transmission of nerve impulses, active transport
In glycolysis, state what one glucose molecule gets broken down into
Two molecules of pyruvate and released enough energy for 2 ATP
State what happens in stage 2 of aerobic respiration
Each pyruvate is broken down to carbon dioxide and water, releasing enough energy to yields a large number of ATP molecules.
How many ATP are produced by fermentation
2 ATP.
What are the products of plant and yeast cell fermentation
The pyruvate molecules are converted to ethanol and carbon dioxide
What is the product of pyruvate in animal cell fermentation
The pyruvate molecules are converted to lactate
Where in the cell does respiration begin
The cytoplasm
Where in the cell is fermentation completed
The cytoplasm
Where in the cell is aerobic respiration completed
The mitochondria
What is fermentation?
The breakdown of glucose in the absence of oxygen to release a small amount of energy
State an effect on your body after fermentation.
Muscle fatigue
What is the name of the equipment used to measure the rate of respiration?
Respirometer
Species
A group of similar organisms which can produce fertile offspring
Define 'biodiversity'.
The variety of species living in a habitat
Define 'population'.
All the organisms of one species in a habitat
What are producers?
Organisms which produce their own food e.g. plants
Define: Consumer
Organisms which rely on eating other organisms for food
Define: Carnivore
A meat-eater(someone who eats animals)
Define: Herbivore
A plant-eater
Define: Omnivore
An organism which eats both plants and animals
Define: Predator
An organism which hunts for food
Define: prey
An organism which is hunted
Define: Food chain
The feeding relationships between a producer and a top consumer
Define: Food web
A network if interconnected food chains in a habitat
What are primary consumers? Give an example.
Animals that eat producers - Any herbivores (eg. cows, sheep, rabbits)
What are secondary consumers? Give an example.
Animals that eat primary consumers - Any carnivores (eg. lions, foxes, eagles)
Secondary consumers may be eaten by...?
Tertiary consumers
Define 'community'.
A group of interdependent organisms containing different species