med term chapter 1

what is biotech

the use of biology to solve human problems

what 3 biological levels can biotech work with

DNA protein and whole organism

what 2 board ancient biotech practices were taught

selective breeding and fermentation

In selective breeding what do humans choose

plants and animals

What key diffrences separates ancient and modern biotech

Modern work with DNA and ancient works with the whole organism

what does recombinant DNA do

they join DNA together

what dose polumrtsdr chain do (PCR)

they copy DNA

what dose DNA sequence do

They Read DNA

what does cloning produce

They match genes

In recombinant insulin production what do bacteria receive

human insulin gene (Plasmid)

what are the main insulin source before biotech

Pig and cow pancreases

what 5 moves trace the successful path through the recombinant insulin diagram

• 1. Cut DNA: Restriction enzymes cut open the bacterial plasmid vector and isolate/cut the human insulin gene.

• 2. Join Plasmid and insulin gene: DNA ligase seals the human gene into the plasmid, forming a recombinant plasmid (ligation).

• 3. Deliver: The recombinant plasmid is introduced into a host bacterium like E. coli through transformation.

• 4. Select: Antibiotic resistance markers or screening methods are used to identify and select only the bacteria that successfully took up the plasmid.

• 5. Grow: The selected recombinant bacteria are cultured and fermented on a large scale to multiply and produce the insulin protein

why are enzymes useful In industrial biotech

because different stains uses different enzymes

what 3 copy traits were targeted by agricultures biotech

pest resistance drought tolerance and enhanced nutritional content

what nutrient deficiency was golden rice designed to address

enhanced nutritional content