VIBRIO, AEROMONAS, HELICOBACTER SPECIES (MODULE 6)

THESE ARE COMMONLY FOUND IN AQUATIC ENVIRONMENTS

GENUS VIBRIO

CURVED OR COMMA SHAPED BACILLIS

A. VIBRIO

B. AEROMONAS

C. HELICOBACTER

D. CAMPYLOBACTER

E. NOTA

A

GRAM NEGATIVE, CURVED OR COMMA SHAPED BACILLI, THEY ARE BASOPHILIC BUT NOT HALOPHILIC

VIBRIO CHOLERAE AND VIBRIO MIMICUS

VIBRIO SPECIES ARE OXIDASE, CATALASE, AND NITRATE REDUCTION POSITIVE EXCEPT FOR WHICH VIBRIO SPECIE?

A. VIBRIO CHOLERAE

B. VIBRIO MIMICUS

C. VIBRIO METSCHNIKOVII

D. VIBRIO VULNIFICUS

E. NOTA

C

REAGENT FOR STRING TEST

0.5% SODIUM DEOXYCHOLATE

IN DIFFERENTIATING VIBRIO AND ENTEROBACTERIA, WHICH IS OXIDASE NEGATIVE?

ENTEROBACTERIA

ALL VIBRIO SPECIES ARE NON-LACTOSE FERMENTER EXCEPT FOR?

A. VIBRIO CHOLERAE

B. VIBRIO MIMICUS

C. VIBRIO METSCHNIKOVII

D. VIBRIO VULNIFICUS

E. NOTA

D

CHEMICAL NAME OF VIBRIOSTATIC COMPOUND (O/129)

2,4-DIAMINO 6,7-DIISOPROPYL PTERIDINE

THESE ARE ACQUIRED FROM CONTAMINATED WATER AND IMPROPERLY STORED FOOD PRODUCTS

VIBRIO CHOLERAE

THE MOST SIGNIFICANT SPECIE OF GENUS VIBRIO

A. VIBRIO VULNIFICUS

B. VIBRIO MIMICUS

C. VIBRIO METSCHNIKOVII

D. VIBRIO PARAHAEMOLYTICUS

E. NOTA

E

WHAT IS THE MOTILITY OF VIBRIO CHOLERAE?

SHOOTING STAR MOTILITY

AGENT OF CHOLERA

VIBRIO CHOLERAE O1

VIBRIO CHOLERAE O1 REACTS WITH WHAT ANTIGEN?

O1 ANTIGEN

AN ENTEROTOXIN RESPONSIBLE FOR THE MASSIVE AMOUNT OF GASTROINTESTINAL WATER AND ELECTROLYTE LOSS

CHOLERA TOXIN/CHOLERAGEN

THIS IS OFTEN THE MOST COMMON CAUSE OF DEATH AMONG PATIENTS WITH CHOLERA

DEHYDRATION

CHOLERA TOXIN IS VERY VIRULENT THAT IT CAN RESULT TO SEVERE DIARRHEA THAT OFTEN RESULTS TO?

A. 20-30 BOUTS/DAY

B. 10-30 BOUTS/DAY

C. 5-10 BOUTS/DAY

D. 10-15 BOUTS/DAY

E. NOTA

B

WHICH BACTERIA CAUSES SUNKEN EYES, WASHERWOMAN’S HANDS AND PALLOR

VIBRIO CHOLERAE O1

WHICH BACTERIA HAS A DIARRHEA THAT IS DESCRIBED TO HAVE RICE WATERY STOOL/PEA SOUP STOOL, WATERY WITH GRAY MUCUS?

A. VIBRIO CHOLERAE O1

B. VIBRIO PARAHAEMOLYTICUS

C. AEROMONAS SPECIES

D. VIBRIO MIMICUS

E. NOTA

A

THE DIARRHEA OF V. CHOLERAE IS DESCRIBED TO BE:

A. RICE WATERY STOOL

B. PEA SOUP STOOL

C. WATERY WITH GRAY MUCUS

D. AOTA

E. NOTA

D

A PATIENT WITH V. CHOLERAE EXHIBITS WHICH OF THE FOLLOWING:

A. DIARRHEA

B. SUNKEN EYES

C. WASHERWOMAN’S HANDS

D. PALLOR

E. AOTA

F. NOTA

E

THE O ANTIGEN PRESENT IN ORIGINAL J

A AND C

THE O ANTIGEN PRESENT IN VARIANT F

A AND B

THE O ANTIGNE PRESENT IN MIDDLE INTERMEDIATE

A, B, AND C

INABA WAS FIRST DISCOVERED IN:

A. JAPAN

B. INDIA

C. PHILIPPINES

D. NOTA

C

OGAWA WAS FIRST DISCOVERED IN:

A. JAPAN

B. INDIA

C. PHILIPPINES

D. NOTA

B

HIKOJIMA WAS FIRST DISCOVERED IN:

A. JAPAN

B. INDIA

C. PHILIPPINES

D. NOTA

A

WHICH OF THE TWO BIOGROUPS OF V. CHOLERAE SHOWS A NON-HEMOLYTIC PATTERN/GAMMA PATTERN?

CLASSIC

WHICH OF THE TWO BIOGROUPS OF V. CHOLERAE SHOWS A BETA-HEMOLYTIC PATTERN?

EL TOR

BIOGROUP OF V. CHOLERAE THAT IS NEGATIVE IN VP TEST, RESISTANT TO POLYMYXIN B, AND NEGATIVE IN CHICKEN RBC AGGLUTINATION

CLASSIC

STRAIN OF V. CHOLERAE THAT PHENOTYPICALLY RESEMBLES O1 BUT DOES NOT REACT WITH O1 ANTIGEN

VIBRIO CHOLERAE NON-O1

WHICH OF THE FOLLOWING IS V. CHOLERAE NON-O1 ASSOCIATED WITH?

A. MILD DIARRHEA

B. EPIDEMIC CHOLERA

C. SKIN/WOUND INFECTION

D. WASHERWOMAN’S HAND

E. NOTA

SELECT ALL THAT APPLIES

A AND C

EMERGING GROUP OF VIBRIO CHOLERAE AS THEY ALSO PRODUCE TOXIN SIMILAR WITH ENTEROTOXIN OF O1

VIBRIO CHOLERAE O139

WHICH OF THE FOLLOWING IS V. CHOLERAE O139 ASSOCIATED WITH?

A. MILD DIARRHEA

B. EPIDEMIC CHOLERA

C. SKIN/WOUND INFECTION

D. WASHERWOMAN’S HAND

E. NOTA

SELECT ALL THAT APPLIES

B

THIS WAS FIRST RECOGNIZED IN JAPAN

VIBRIO PARAHAEMOLYTICUS

VIBRIO PARAHAEMOLYTICUS IS AN AGENT OF ______ IN JAPAN

SUMMER DIARRHEA

THIS IS THE 2ND MOST COMMON VIBRIO SPP. ASSOCIATED WITH GASTROENTERITIS

VIBRIO PARAHAEMOLYTICUS

THIS IS ACQUIRED FROM SALT WATER AND CONTAMINATED MARINE ANIMALS

A. VIBRIO PARAHAEMOLYTICUS

B. VIBRIO CHOLERAE

C. VIBRIO ALGINOLYTICUS

D. VIBRIO VULNIFICUS

E. NOTA

A

WHAT DOES V. PARAHAEMOLYTICUS EXHIBIT THAT MAKES THEM UNIQUE FROM OTHER VIBRIO SPECIES?

KANAGAWA PHENOMENON

KANAGAWA PHENOMENON SHOWS BETA-HEMOLYTIC ONLY IN WHAT MEDIA?

WAGATSUMA AGAR/MEDIA

TRUE OR FALSE. WAGATSUMA AGAR/MEDIA CONSIST OF BLOOD AGAR PLATE WITH HIGH SALT CONCENTRATION AND SUCROSE

FALSE

KANAGAWA PHENOMENON OCCURS DUE TO?

THERMOSTABLE DIRECT HEMOLYSIN/HEAT STABLE HEMOLYSIN

AGENT OF TYPE 1 NF

VIBRIO VULNIFICUS

VIBRIO VULNIFICUS IS CLASSIFIED AS WHAT BACTERIA?

FLESH EATING BACTERIA

THIS MAY ALSO CAUSE SEPTICEMIA THROUGH CONSUMPTION OF CONTAMINATED SHELLFISH

VIBRIO VULNIFICUS

STUDIES HAVE SHOWN THAT V. VULNIFICUS ARE ALSO RECOVERED IN?

TILAPIA

THIS IS THE LEAST PATHOGENIC VIBRIO SPECIES AND MAY ONLY OCCUR AS OPPORTUNISTIC PATHOGEN

VIBRIO ALGINOLYTICUS

WHICH OF THE FOLLOWING IS V. ALGINOLYTICUS ASSOCIATED WITH?

A. EYE INFECTION

B. EAR INFECTION

C. BURN/WOUND INFECTION

D. DIARRHEA

E. NOTA

SELECT ALL THAT APPLIES

A, B, C

WHICH OF THE FOLLOWING ARE USUALLY INFECTED BY V. ALGINOLYTICUS

A. FISHERMAN

B. LIFEGUARDS

C. SAILORS

D. AOTA

E. NOTA

SELECT ALL THAT APPLIES

A AND C

SPECIMEN FOR VIBRIO SPECIES

STOOL AND RECTAL SWAB

WHEN SPECIMEN IS RECTAL SWAB, IT SHOULD BE TRANSPORTED USING AN APPROPRIATE TRANSPORT MEDIA TO PREVENT ________ OF THE RECTAL SWAB

DESSICATION OR DRYING

WHAT IS THE TRANSPORT MEDIA FOR RECTAL SWAB SPECIMEN?

CARY BLAIR

THIS IS NOT RECOMMENDED AS TRANSPORT MEDIA FOR VIBRIO

BUFFERED GLYCEROL SALINE

WHAT SHOULD BE AVOIDED AS IT CAN BE TOXIC TO VIBRIO?

GLYCEROL

ENRICHMENT MEDIA FOR VIBRIO SPECIE

ALKALINE PEPTONE WATER

ALKALINE PEPTONE WATER IS ENRICHED WITH?

SODIUM CHLORIDE

SELECTIVE-DIFFERENTIAL MEDIA FOR VIBRIO SPECIES?

THIOSULFATE-CITRATE-BILE SALTS-SUCROSE AGAR

PH INDICATOR USED IN TCBS AGAR

BROMTHYMOL BLUE

V. PARAHAEMOLYTICUS SHOWS WHAT COLOR IN TCBS AGAR?

GREEN COLOR

______ IS A NON-SPECIFIC TEST FOR ______

CHOLERA RED REACTION; VIBRIO CHOLERAE

REAGENT FOR CHOLERA RED REACTION

SULFURIC ACID

POSITIVE REACTION FOR CHOLERA RED REACTION

RED COLOR

CHOLERA RED REACTION SHOWS RED COLOR DUE TO THE PRESENCE OF?

NITROSO INDOLE

A BACTERIA MAY SHOW FALSE POSITIVE IN CHOLERA RED REACTION IF:

A. INDOLE TEST POSITIVE

B. NITRATE REDUCER

C. OXIDASE POSITIVE

D. UREASE POSITIVE

E. NOTA

SELECT ALL THAT APPLIES

A AND B

THESE BACTERIA ARE NATURALLY FOUND IN FRESH AND SEA WATER. THEY ARE ALSO FOUND IN DRAINS, SINK TRAPS, DISTILLED AND TAP WATER

GENUS AEROMONAS

THESE ARE GRAM NEGATIVE FACULTATIVE ANAEROBIC RODS, INDOLE AND DNASE POSITIVE

GENUS AEROMONAS

GENUS AEROMONAS USUALLY SHOWS BETA-HEMOLYTIC ON BAP EXCEPT FOR?

AEROMONAS CAVIAE

GENUS AEROMONAS ARE MOTILE EXCEPT FOR?

AEROMONAS SALMONCIDA

TRUE OR FALSE. IN CIN MEDIA, AEROMONAS SPECIES ARE INDISTINGUISHABLE WITH YERSINIA PESTIS

FALSE

YERSINIA ENTEROCOLITICA AND AEROMONAS SPECIES SHOWS WHAT APPEARANCE IN CIN MEDIA?

BULLSEYE APPEARANCE

THIS IS SAID TO BE RESISTANT TO 2,4-DIAMINO 6,7-DIISOPROPYL PTERIDINE

GENUS AEROMONAS

THE OPTIMAL GROWTH TEMPERATURE OF MESOPHILIC GROUP

37C

THEY ARE MOTILE AND ARE ALL ASSOCIATED WITH GASTROENTERITIS AMONG HUMANS

MESOPHILIC GROUP

THEY ARE NON MOTILE AND ARE USUALLY NOT HUMAN PATHOGEN BUT RATHER FISH PATHOGENS

PSYCRHOPHILIC GROUP

OPTIMAL GROWTH TEMPERATURE OF PSYCHROPHILIC GROUP

22C

IT IS THE MOST COMMON HUMAN ISOLATE IN THE GENUS AEROMONAS THAT IS ASSOCIATED WITH GASTROENTERITIS

AEROMONAS HYDROPHILA COMPLEX

THE ONLY AEROMONAS SPECIE UNDER PSYCHROPHILIC GROUP

AEROMONAS SALMONCIDA

GRAM NEGATIVE PLEOMORPHIC RODS WHICH MAY APPEAR FILAMENTOUS, THEY ARE NON-HEMOLYTIC AND ARE INOSITOL FERMENTER

PLESIOMONAS SHIGELLOIDES

THEY ARE ASSOCIATED WITH SECRETORY DIARRHEA AMONG HIV PATIENT WITH INFLAMMATORY BOWEL SYNDROME/DISEASE

PLESIOMONAS SHIGELLOIDES

PLESIOMONAS SHIGELLOIDES CAN CAUSE OTHER INFECTIONS BESIDES INFLAMMATORY BOWEL DISEASE SUCH AS?

GASTROENTERITIS AND SEPTICEMIA

TEST TO DIFFERENTIATE VIBRIO FROM AEROMONAS

O/129 SUSCEPTIBILITY

TEST TO DIFFERENTIATE VIBRIO FROM PLESIOMONAS SHIGELLOIDES

INOSITOL FERMENTATION

THEY ARE FAINTLY GRAM NEGATIVE COMMA, CURVED, S SHAPED, SEAGULL WING SHAPED BACILLI

CAMPYLOBACTER

RECOMENNDED COUNTERSTAIN FOR CAMPYLOBACTER

CARBOL FUCHSIN

IF SAFRANIN IS STILL USED AS A COUNTERSTAIN IN CAMPYLOBACTER, WHAT SHOULD YOU DO?

EXTEND THE COUNTERSTAINING FOR 2-3 MINUTES

IN ORDER TO PROMOTE THE GROWTH OF CAMPYLOBACTER WE USED?

CANDLE JAR

MOTILITY OF CAMPYLOBACTER

DARTING MOTILITY

TRUE OR FALSE. DARTING MOTILITY OF CAMPYLOBACTER CAN ONLY BE OBSERVED IN HANGING DROP METHOD

TRUE

WHICH BACTERIA IS OXIDASE POSITIVE, UREASE NEGATIVE, AND NON-FERMENTATIVE

CAMPYLOBACTER

THIS CAUSES GASTROENTERITIS AND DIARRHEA

CAMPYLOBACTER JEJUNI

THE MOST COMMON CAUSE OF BACTERIAL GASTROENTERITIS WORLDWIDE

CAMPYLOBACTER JEJUNI

THIS BACTERIA WAS KNOWN TO BE THE “GLOBAL DIARRHEA”

A. CAMPYLOBACTER FETUS SUBSP FETUS

B. CAMPYLOBACTER PYLORI

C. CAMPYLOBACTER JEJUNI

D. VIBRIO CHOLERAE

E. NOTA

C

CAMPYLOBACTER JEJUNI IS ASSOCIATED WITH A CONDITION CALLED?

GUILLAIN-BARRE SYNDROME

ALL ARE ENTERIC CAMPYLOBACTER EXCEPT:

A. CAMPYLOBACTER JEJUNI

B. CAMPYLOBACTER PYLORI

C. CAMPYLOBACTER COLI

D. CAMPYLOBACTER LARI

E. NOTA

B

CAMPYLOBACTER SPECIE THAT IS ISOLATED FROM BLOOD CULTURE

CAMPYLOBACTER FETUS SUBSP FETUS

THIS RARELY CAUSES GASTROINTESTINAL ILLNESS AS IT IS AN OPPORTUNISTIC PATHOGEN

CAMPYLOBACTER FETUS SUBSP FETUS

SPECIMEN USED IN DIAGNOSING CAMPYLOBACTER SPECIES

STOOL, RECTAL SWAB, BLOOD

WHAT COMPONENTS DOES MODIFIED SKIRROW’S MEDIA HAVE?

BLOOD AGAR PLATE (7% HORSE BLOOD) WITH VANCOMYCIN, TRIMETHOPRIM, POLYMYXIN B

WHAT COMPONENTS DOES CAMPY BAP HAVE?

BRUCELLA AGAR WITH 10% SHEEP’S BLOOD, TRIMETHOPRIM, POLYMYXIN B, CEPHALOTHIN, VANCOMYCIN, AMPHOTERICIN B

CVA MEANS?

CEFOPERAZONE VANCOMYCIN AMPHOTERICIN B

THIS SELECTIVE CULTURE MEDIA PROVIDES BETTER INHIBITION OF FECAL FLORA HENCE IMPROVING THE RECOVERY OF CAMPYLOBACTER IN STOOL SAMPLE

CAMPY CVA