Microbiology Media Lab

Streptococcus anginosus odor

sweet, butterscotch or honeysuckle odor

Pseudomonas aeruginosa odor

fruity or grape odor

Proteus mirabilis odor

burnt chocolate odor

Eikenella corrodens odor

bleach-like

Cystine-tellurite agar is used for the isolation of ___

Corynebacterium diphtheriae

On the cystine-tellurite agar, Corynebacterium colonies are ___ due to the reduction of tellurite

black

The slide coagulase test is based on the detection of ___ coagulase that is produced on the bacterial cell well.

bound

Buffered Charcoal Yeast Extract (BCYE) agar is the ___ solid culture medium used for the primary recovery of ___.

nonselective; Legionella pneumophila

Kock-Weeks bacillus is also known as ___

Haemophilus aegyptius

Haemophilus influenzae requires factor ___ (hemin) and factor ___ (NAD).

X; V

The Satellitism test consists of Haemophilus influenzae growth near ___ ___ on a blood agar

Staphylococcus aureus (H. flu needs help to grow - so it follows staph’s glow!)

The satellite test for the identification of Haemophilus influenzae works because:

Blood agar has factor X, filling one out of the two needs for H. flu growth. But when plated with S. aureus, H. flu then receives the factor V it needs.

Taenia solium is the ___ tapeworm, caused by ingestion of raw or undercooked meat where the ___ (head) is passed into the small intestine to attach and mature.

pork; scolex

The Taenia solium scolex has ___ suction-cup-like suckers with hooklets

four

Is Entamoeba hartmanni a pathogenic or nonpathogenic organism?

Cysts are typically small (___), containing ___ nuclei.

nonpathogenic; 5-10 um; four

Entamoeba coli is the only species found in humans that has a cyst stage containing more than ___ nuclei.

They are typically larger than other intestinal amoebae (___).

four; 10-35 um

Entamoeba histolytica is a ___ pathogen, their cysts measure up to (___) with ___ nuclei

major; 10-20 um; four

Bordet-Gengou plates are used to isolate…

Bordetella pertussis

___ tuberculosis is commonly isolated by Lowenstein Jensen (L-J) media

Mycobacterium

Corynebacterium ___ is commonly isolated by cystine-telluride blood agar or modified Tinsdale agar (TIN).

diphtheriae

Haemophilus ___ is commonly isolated using chocolate agar, as it provides both hemin (X factor) and NAD (V factor)

influenzae

The ___ species have erect sporangiospores with brown rhizoids that anchor cells

Rhizopus

Iodine mounts are helpful to visual the internal structures of parasites, such as protozoan ___.

cysts

The gametocytes of Plasmodium falciparum are the only malarial organisms that assume a characteristic ___ shape.

banana

Edwardsiella tarda is associated with what animal?

reptiles (Edward Cullen and reptiles are cold-blooded!)

In a urine culture, a colony count of <___ does not correlated with clinical infection- organism should be listed as a ___ ___ due to the low colony count.

10,000; possible contaminant

Trichuris trichiura (whipworm) eggs are 50-55 um by 20-25 um; ___-shaped; thich-shelled, and posses a pair of polar “___” at each end

barrel; plugs

Schistosoma spp. eggs have specific ___ to differentiate between them

Identify which organism has the: small, rudimentary lateral spine; lateral spine; and terminal spine

S. mansoni
S. haematobium
S. japonicum

spines

S. mansoni = lateral
S. haematobium = terminal
S. japonicum = small, rudimentary lateral

To determine if Penicillin is susceptible or resistant, Penicillin-resistant strains of Streptococcus pneumoniae can be determined if a zone size of = 20 mm with the oxacillin disk screen is confirmed with an ___ ___ ___ (MIC) method

Minimum Inhibitory Concentration

The polysaccharide capsule is the primary virulence factor produced by Streptococcus ___ that protects the organism from ___

pneumoniae; phagocytosis

___ is caused by infection with Treponema pallidum

Syphilis

Treponema pallidum can be detected by the detection of the ___ gene.

poIA

___ ___ is the leading cause of meningitis and pneumonia with or without bacteremia

Streptococcus pneumoniae

A positive ___ coagulase test establishes a definitive identification of Staphylococcus aureus

tube

Haemophilus ___ is the causative agent of chancroid, a highly contagious sexually transmitted genital ulcer disease

ducreyi

Bordetella bronchiseptica is an agent causing ___ tract infections in animals; not transmitted by wound infection

respiratory

Staphylococcus intermedius, pasteurella multicida, and Capnocytophaga cynodegmi are all associated with dog ___

bites