Exam 3: Bacterial Transcription

What are the Major players of Bacterial transcription

Sigma factor, promotor sequence, and RNA polymerase

What is the Sigma Factor

it’s a part of bacterial RNA pol. that recognizes the promoter sequence on DNA

What is the promoter sequence

it’s the recognition site for the sigma factor to bind to

Where are promoters found?

They are always found upstream of transcription start site

What is holoenzyme

RNA pol. + sigma factor

What is initiation

When the RNA polymerase initiates Transcription at the promoter site

What are the two sites the sigma factor can recognize

the -35 sequence and the -10 sequence

How does the sigma factor bind to the -35 sequence? the -10?

To the -35 sequence it binds loosely, to the -35 sequence it binds more tightly to the -10 sequence

What are the -35 and -10 sequence examples of

consensus sequences

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What is elongation

when the RNA pol. adds RNA nucleotides to the RNA strand

What is termination

the ending and release of both polymerase and complete RNA transcript