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Eukaryote
A cell that contains a nucleus and membrane bound organelles
Prokaryote
A unicellular organism that lacks a nucleus and membrane bound organelles
Ubiquitous
Present or existing everywhere
Photosynthesis
- Conversion of light energy from the sun into chemical energy.
- Light fueled conversion of CO2 into organic material.
Anoxygenic Photosynthesis
No oxygen produced in photosynthesis
Genetic Engineering
Manipulation of genetic microbes, plants, and animals to modify organisms
Recombinant DNA technology
The transfer of genetic material from one organism to another to deliberately alter the DNA and produce a specific product.
Pathogen
- Infectious agent
- Causes disease or illness
Sterile
Completely free of all life forms, including spores and viruses
Louis Pasteur
- A French chemist, this man discovered that heat could kill bacteria that otherwise spoiled liquids including milk, wine, and beer.
- Introduced the germ theory.
- Began pasteurizing milk to kill bacteria.
- Contributed to the understanding of microbial role in wine, beer, and milk.
Robert Koch
- Developed the culture plate method to identify pathogens.
- Verified the germ theory.
Six major groups of microorganisms
- Bacteria
- Helminths (worms)
- Protozoa
- Fungi
- Algae
- Viruses ( can infect all of the above)
Helminth
A parasitic worm (Ex. Tapeworm, Liver fluke, Ascarid, or Leech).
Immunology
Study of host response to infection
Epidemiology
- Branch of medical science concerned with the incidence, distribution, and control of diseases that affect large numbers of people.
- Study of community health.
Geomicrobiology
Study of microbes in the earth's crust
Biotechnology
- A form of technology that uses living organisms, usually genes, to modify products, to make or modify plants and animals, or to develop other microorganisms for specific purposes.
- The use of microbes of their products for commercial or industrial purposes.
Virus
- Acellular microbes that are comprised of nucleic acid surrounded by a protein coat.
- Uses an infected host cells genetic and metabolic machinery for reproduction and assembly.
- Nonliving agents that require a host cell to complete their life cycle.
Binomial System of Nomenclature
Scientific name combination starting with the generic name and ending with the species name
Archaea
- Domain of unicellular prokaryotes that have cell walls that do not contain peptidoglycan.
- Prokaryotic single celled organism of primitive origin that are capable of living in extreme or very harsh environments.
Peptidoglycan
A protein-carbohydrate compound that makes the cell walls of bacteria rigid.
Whittaker system of classification
- Kingdoms based on cell structure and type, body organization and nutritional type
~ Plantae
~ Protista
~ Monera
~ Fungi
~ Animalia
Levels of classification (largest to smallest)
~ Domain
~ Kingdom
~ Phylum
~ Class
~ Order
~ Family
~ Genus
~ Species
Taxonomy
The science of classifying organisms
Woese fox system of classification
Domain eukarya, domain bacteria, domain archaea
Biofilm
~ A surface-coating colony of one or more species of prokaryotes that engage in metabolic cooperation.
~ Accumulation of bacteria and other microbes on surfaces
Holmes and Semmelweis
~ Showed that women became infected in the maternity ward after examinations by physicians who had been working in the autopsy rooms.
~ Two physicians who recognized that humans can transfer infections to each other.
T/F: Scientific names are italicized and printed documents.
True
Nomenclature
Assignments of scientific names to various taxonomic categories and individual organisms.
Varion
Virus particle that is not inside of a host cell
Bioremediation
Introducing microbes into the environment to restore stability or to clean up toxic pollutants (ex. oil spills)
Staphylococcus belongs to?
Domain bacteria
Tyndall & Cohn
Two scientists who discovered heat resistant forms of bacteria called endospores
Joseph Lister
English surgeon who was the first to use antiseptics/aseptic technique. (1827-1912)
Inductive reasoning
A type of logic in which generalizations are based on a large number of specific observations.
Deductive reasoning
Reasoning in which a conclusion is reached by stating a general principle and then applying that principle to a specific case (The sun rises every morning; therefore, the sun will rise on Tuesday morning.)
Restriction Enzymes
- Enzyme that cuts DNA at a specific sequence of nucleotides.
- Allowing microbiologists to isolate specific pieces of DNA for transfer into other cells.
Polymerase Chain Reaction (PCR)
- A technique for amplifying DNA in vitro by incubating with special primers, DNA polymerase molecules, and nucleotides.
- Allows us to amplify tiny amounts of DNA, creating a powerful tool for Dx, forensics, and microbial investigation.
Spontaneous Generation
Hypothesis stating that life could arise from nonliving matter.
Bacteriology
Study of prokaryotes (bacteria and archaea)
Mycology
Study of fungi
Phycology
Study of algae
Protozoology
Study of protozoa
Virology
Study of viruses
Immunology
Study of immune health/the immune system
The rule of ___ can be paraphrased as "like dissolves like"
Solubility
___________ ________ are the building blocks of proteins and they exist in ____ different naturally occurring forms.
Amino acids ; 20
Four levels of protein organization
1. Primary
2. Secondary
3. Tertiary
4. Quaternary
Pure water in considered neutral since it has a pH of
7
-7 is the neutral # for pH, the higher the more basic and the lower the more acidic
What macromolecule exemplified by glycogen, is involved in the energy storage, nutrient storage, structural support and protection?
Carbohydrates
________________ is a long, fibrous polymer composed of glucose found in plants and algae.
Cellulose
_______ is a polysaccharide found in seaweed that is used to make solid culture media.
Agar
A polymer of glucosamine that makes up the exoskeletons of certain fungi is called
Chitin
_____________________ is a network of polysaccharide chains cross-linked by short peptides that forms the structural support of bacterial cell walls.
Peptidoglycan
Peptides
Short chains of amino acids that can function as neurotransmitters or hormones.
Polysaccharides
Carbohydrates that are made up of more than two monosaccharides
Monosaccharides
Single sugar molecules (simple sugars)
Carbohydrates function
main source of energy (short term)
carbohydrates, lipids, proteins, nucleic acids
4 macromolecules
Lipids
Energy-rich organic compounds, such as fats, oils, and waxes, that are made of carbon, hydrogen, and oxygen.
Proteins
Nutrients the body uses to build and maintain its cells and tissues
Chains of amino acids
_______ in gram-negative bacterial cell walls is also known as endotoxin, and it causes fever and shock in the host.
Lipopolysaccharide
The outer coating of polysaccharides found on some bacteria that functions in the attachment to other cells, protection or communication is called the ________________.
Glycocalyx
____________, like cellulose, is a polymer of glucose but in this case, it is a branching chain.
Starch
___________ is a glucose polymer stored by cells.
Glycogen
Lipids encompass which four of the following groups of compounds?
Steroids, Waxes, Phospholipids, Triglycerides
Triglycerides are composed of a single molecule of _________ bound to three ________ ________.
Glycerol ; Fatty Acids
_________ ________ are long hydrocarbon molecules with a carboxyl group at one end.
Fatty Acids
__________ fats have hydrocarbon chains with at least one double bond.
Unsaturated
solid fats are more _____ than liquid oils.
Saturated
When triglycerides are acted on by lipases, the _______ and ______ are freed to be used in metabolism.
Fatty Acids ; Glycerol
Fatty Acids
Chains of carbon atoms bonded to hydrogen atoms that make up most lipids
Glycerol
A three-carbon alcohol to which fatty acids are covalently bonded to make fats and oils.
_________ _______ are the type of macromolecules that yield the most energy per molecule.
Fatty acids
Phospholipids have a _____ charged head and a _____ uncharged tail.
Hydrophilic, Hydrophobic
_____________ bilayer formation is significant in membrane formation and contributes to the selective permeability and fluid nature of membranes.
Phospholipid
A membrane is composed primarily of ________ & ________.
Lipids and Proteins
___________ are a class of ring shaped compounds that are commonly identified as animal hormones and cell membrane components in animals and fungi.
Steroids
Which molecules functions to reinforce the membranes of animal cells?
Cholesterol
Cholesterol
A lipid that forms an essential component of animal cell membranes and acts as a precursor molecule for the synthesis of other biologically important steroids.
What is NOT a characteristic of wax?
Complex ringed compound
___________ are the predominant organic macromolecules in cells and are responsible for their structure, behavior, and unique qualities.
Proteins
Proteins are polymers composed of building blocks called __________ _______.
Amino Acids
Various combinations composed of amino acids make up a nearly infinite variety of __________.
Proteins
The _______________ bond is a ________________ bond that forms between the two amino group of one amino acid and the carboxyl group on another amino acid.
Peptide
Peptide
Short chain of amino acids linked by peptide bonds
The type, number, and order of amino acids in the chain describe its ____________ structure.
Primary
The formation of hydrogen bonds that result in the formation of alpha-helices or beta-sheets constitutes a protein's _______ structure.
Secondary
A protein's ___________ structure is the most complex protein structure and is characterized as the overall three-dimensional shape of a large protein composed of multiple polypeptide chains.
Quaternary
The complete set of genetic information for a cell is carried in a molecule called
DNA
The nucleic acid responsible for carrying out the hereditary program transmitted by an organism's DNA is called
RNA
Components of a nucleotide
Phosphate group, 5 carbon (pentose) sugar, Nitrogenous base
Nucleotide
A building block of DNA, consisting of a five-carbon sugar covalently bonded to a nitrogenous base and a phosphate group.
DNA & RNA are made up of repeating monomers called _________.
Nucleotides
The backbone of DNA is a chain of altering ____________ and ___________ molecules.
Alternating sugar & phosphate molecules
mRNA (messenger RNA)
The form of RNA which is created as a blueprint from DNA; carries instructions for making a protein
tRNA (transfer RNA)
Type of RNA molecule that transfers amino acids to ribosomes during protein synthesis
rRNA (ribosomal RNA)
Type of RNA that makes up the major part of ribosomes
ATP is a molecule containing the nitrogenous base __________, ribose, and 3 phosphates.
Adenine
Polysaccharides contribute to, serve, or serve as
- Nutrient storage
- Structural Support
- Energy stores
- Protection