cell and molecular biology midterm

Explain PCR simply - list B

Polymerase chain reaction has three steps, in denaturing the multi strand DNA separates into single strand. In annealing the primers bind to a target sequence. Finally in extension a new strand of DNA is built

Explain DNA packing simply - List A

DNA wraps around histones, nucleosomes fold into thick fiber which forms loops, the DNA becomes super dense

Explain RNA processing simply - List A

RNA processing modifies and edits pre-mRNA so it becomes mature mRNA that can safely leave the nucleus and be translated into protein.

Explain immunofluorescence - List B

Immunofluorescence uses fluorescently labeled antibodies to bind and visualize specific proteins inside cells under a special microscope.

cells vary in

size, shape and function

cells share

same molecular bases

chromosomes form

territories in the nucleus

chromatin states regulate

gene activity

New DNA Strand Is Synthesized only in

5’-to-3’ Direction

RNA folding is done by

Internal Base Pairing

A Master Transcription Regulator Can

Direct the Formation of An Entire Organ

Nearly ____ of the human genome
contains snippets of viral DNA left over
from ancient infections

one tenth

Automated Dideoxy Sequencing Using
Uniquely Colored Fluorescent

ddNTPs

Techniques Are
Powerful Tools to Study Gene Function

Recombinant DNA