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Trend
A directional relationship between two continuous variables.
Linear regression
A statistical method used to test whether a linear trend exists between two continuous variables.
Regression equation
y=mx+c where m is slope and c is intercept.
Slope (m)
The amount by which Y changes for each unit increase in X.
Intercept (c)
The value of Y when X = 0.
Null hypothesis for trends
There is no trend; slope = 0 ( y=C ).
Alternative hypothesis for trends
There is a trend; slope ≠ 0 ( y=mx+C ).
F‑statistic
Test statistic used in linear regression to determine whether the slope is significantly different from zero.
Significance F
The p‑value for the regression model; shows whether the trend is statistically significant.
R² (coefficient of determination)
Proportion of variation in Y explained by X.
High R²
Strong relationship; X explains much of the variation in Y.
Low R²
Weak relationship; X explains little of the variation in Y.
Residuals
Differences between observed and predicted Y values.
Best‑fit line
The regression line that minimises the sum of squared residuals.
Prediction using regression
Substituting X into the regression equation to estimate Y.
Standard error (SE)
Measure of uncertainty in an estimate (e.g., mean or slope).
Standard error of the mean (SEM)
SEM=sn where s is sample standard deviation.
Confidence interval (CI)
Range of values likely to contain the true population parameter.
95% confidence interval
Mean ± 1.96 × SEM.
Effect of sample size on SEM
Larger sample size → smaller SEM → more confidence.
Normal distribution
Bell‑shaped distribution describing many biological variables.
68–95–99.7 rule
68% of values within 1 SD, 95% within 2 SD, 99.7% within 3 SD.
Error bars
Graphical representation of variability (often SEM or CI).
Purpose of regression
To test whether X predicts Y and quantify the strength of the relationship.
Example trend (lecture)
Higher GDP per capita is associated with lower child mortality.
Interpreting slope sign
Negative slope → Y decreases as X increases; positive slope → Y increases as X increases.
Interpreting p‑value in regression
p < 0.05 → reject H₀ → significant trend.