Detection of Protein Oxidation

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6 Terms

1
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Why is protein oxidation hard to detect?

It is unstable and artificial oxidation occurs during lysis

2
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Why is acid lysis used?

Low pH keeps cysteines protonated and reduced

3
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What chemical selectively binds reduced cysteine?

Cysteine-binding reagents

4
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How does DTT help detect disulfides?

It reduces disulfide bonds

5
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What indicates oxidation in DTT-sensitive assays?

Shift from dimer to monomer

6
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What are limitations of disulfide detection?

Mixed disulfides and need for specific antibodies