Gene Cloning

0.0(0)
Studied by 0 people
call kaiCall Kai
Locked
learnLearn
examPractice Test
spaced repetitionSpaced Repetition
heart puzzleMatch
flashcardsFlashcards
GameKnowt Play
Card Sorting

1/4

encourage image

There's no tags or description

Looks like no tags are added yet.

Last updated 2:34 AM on 6/26/26
Name
Mastery
Learn
Test
Matching
Spaced
Call with Kai
Chat

No analytics yet

Send a link to your students to track their progress

5 Terms

1
New cards

Insert/Chromosomal DNA

DNA fragment of interest for cloning. Obtained from PCR

2
New cards

Cloning Vector/Vector DNA

Vehicle that transports foreign material into DNA. Normally plasmids. Contains an origin of replication (site of DNA replication), MCS (unique sites for restriction enzymes to cut), and markers (identifies cells with the vector)

3
New cards

Restriction Enzymes

Proteins that cut DNA at specific sequences. They are essential for creating recombinant DNA by processing the insertion of foreign DNA into cloning vectors. Forms sticky and blunt ends by cutting target gene and vector.

4
New cards

DNA Ligase

The molecular glue that brings together the target gene and vector by catalyzing formation of phosphodiester bonds

5
New cards

Host Cells

Living cells that are the factory for the vector