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protists
more structural and functional diversity than all other euk clades, most are unicellular, complex anatomy with organelles in single cells,
plastid origins via endosymbiosis
primary and secondary endosymbiosis (two different ways of evolution)
primary endosymbiosis
eukaryote engulfs a bacterium
primary endosymbiosis example
heterotrophic eukaryote engulfing cyanobacteria to create plastid lineages, bacterium engulfed by archaean cell to create mitochondria
secondary endosymbiosis
a eukaryotic cell engulfs another eukaryotic cell which has already undergone primary endosymbiosis
secondary endosymbiosis example
red or green algae being engulfed by a eukaryote
excavata clade
cytoskeleton, some have an excavated feeding groove, uncludes modified mitochondria, unique flagella
organisms in excavata
diplomonads, parabasilids, euglenids
diplomonads
reduced mitochondria (mitosomes), live in anaerobic environments and get energy via biomechanical pathways, have two nuclei, multiple flagella, often parasites
giardia intestinalis
diplomonad, global, found in water, most common infection of intestines, deadly in kids (dehydration)
parabasilids
reduced mitochondria (hydrogenosomes-derive some energy anaerobically)
trichomonas vaginalis
causes yeast infections, parabasilid
euglenozoans
includes predatory heterotrophs, photosynthetic autotrophs, mixotrophs, parasites, have a spiral, crystal rod within flagella, includes kinetoplastids and euglenids
kinetoplastids
type of euglenozoan, trypanosomes, cause sleeping sickness in aftrica and chagas disease in america
euglenids
mixotrophic, can change morphology while moving, no cell wall, contractile vacuole, very motile, have a pellicle (saw them in lab)
sar clade
stramenopiles, includes diatoms, brown algae, golden algae, most have two flagella-one hairy and one smooth
diatoms
important in global carbon cycle, a major component of marine phytoplankton, unique, two part cell wall made of silicon dioxide
golden algae
named for their unique color which comes from different pigments that mask chlorophylls, mostly biflagellate, all primary producers, some mixotrophs, mostly unicellular but some are colonial
dinobryon
golden algae, colonial, freshwater
brown algae
most common algae, multicellular, “seaweed,” contain a holdfast, stipe, and blades
giant kelps
brown algae example
alternation of generations
seen in some multicellular algae and most plants, alternating between multicellular forms of haploid and diploid structures
prokaryotic benefits to humans
biological treatment of wastewater, recover metal from ores, synthesis of vitamins, production of antibiotics and hormones
bacterial ecological functions
nitrogen fixation
eutrophication
excess n and p sent to surface of water, leads to cyanobacteria blooms, cause bad water quality and eventually ead to anoxic conditions (low o2 levels kills fish)
biotech uses of prokaryotes
bioremediation (clean up oil spills, plastic degradation), bioplastic synthesis, crispr
nitrogen fixation
the conversion of n2 into biologically available forms of nitrogen
transduction
phage infects bacteria, its dna gets packaged into new virus, transferred after cell lysis to a new host
conjugation
process where genetic material from one cell is sent to another via a sex pilus
conjugation example
f plasmid in e coli
transformation
cells pick up dna from their surroundings and encorporate it into their genome
three types of horizontal gene transfer
conjugation, transduction, transformation
evidence of rapid prokaryotic evolution
richard lenski (msu) tested e coli’s adaption to a new environment, found that they can evolve rapidly to adapt to a new environment
genetic recombination
combining of dna from two different sources
protocells
early protocells with self replicating catalytic rna were more efficient therefore increased in number, ribozymes allowed for replication, implies that early life was ikely an “rna world” and that rna could have provided the template for making dna
ribozymes
ribonucleic acid enzymes, a catalytic form of rna
history of life on earth
4 bya-isotopic evidence of life signatures, 3.5 bya- stromatolites, cyanobacterial mats
problems with fossil records
hard to find places where fossils form, favors hard skeletons/shells (no bacteria), favor organisms that existed for a long time, common species fossilized more, widespread distribution
current atmosphere composition
n2-79, o2-20-21, ar- less than 1, co2-430 ppm (plus h20)
first oxygen revolution
2.3 bya, prokaryotes (specifically cyanobacteria), then unicellular eukarya
second oxygen revolution
800 mya, evolution of plants (marine and terrestrial)
cambrian explosion
540-525 mya, lots of vertebrae and new diversity, includes chordae (fish)
permian extinction
251 mya, volcanism increased co2, lead to ocean acidification, boundary between paleozoic and mesozoic ages
asteroid at mexico
boundary between mesozoic and cenozoic, caused great extinction
extinction rates
increase with temperature increases
sixth great extinction
right now
prokaryotes
found in all major habitats, huge genetic diversity, most are unicellular, no membrane bound organelles
cocci
spherek, 1 um
bacilli
1 um, oval
spirelli
long squiggle, 3 um
strata/stratigophy
relative ages only in geologic layers of “older” and “newer” sedimentary rock
radioisotopes
used for absolute dating and aging of rocks and fossils
endosymbiotic theory
lynn margulis et al, mitochondria and other plastids were put together like independent prokaryotic cells and that eukaryotic organelles were previously free living prokaryotic organisms
serial endosymbioses
eukarya developed via multiple endosymbioses, including dna and reproduction in bacteria and mitochondria
gram positive
thick wall of peptidoglycan
gram negative
thin wall of peptidoglycan and covered by a layer of lipopolysaccharides (often toxic)
bacterial features
endospores, capsules, fimbrae
archaea cell walls
lack peptidoglycan
peptidoglycan
a network of sugar polymers with polypeptides linking them
bacterial motility
prokaryotic flagellum, chemotaxis
positive taxis
movement toward stimulus
negative taxis
movement away from stimulus
photoautotroph
energy source is light, carbon source is co2 or hco3c
chemoautotroph
energy source is inorgamic chemicals, carbon source is co2 or hco3
photoheterotroph
energy source is light, carbon source is organic compounds
chemoheterotroph
energy source and carbon source is organic compounds