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Isotonic
________- this means that the solution contains the correct levels of dissolved salts and sugars.
ultracentrifuge spins
As the ________, centripetal force pushes the organelles with the most mass to the bottom of the container.
what is the function of a buffer?
regulates the pH of the solution.
What is cell fractionation?
the process used to separate cellular components while preserving their individual functions.
Why is the solution cold?
to reduce enzyme activity
What is "isotonic"?
the solution contains the correct levels of dissolved salts and sugars
Buffered
organelles may die or function inefficiently in certain pH values
Why is the solution isotonic?
prevents water moving in or out of cells by osmosis, preventing organelles from shrivelling up or bursting
Why is the solution buffered?
some organelles may die or stop functioning at certain pH values
What force does the ultracentrifuge use?
Centripetal force
How does the ultracentrifuge work?
Organelles with the greatest mass are pushed the furthest away from the centre, to the bottom of the test tube. Organelles are separated by mass.
Sediment
Concentrated mass of the heaviest part of the sample, sinks to the bottom
Supernatant
the clear liquid that lies above a sediment, containing all the other organelles
Note 
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