Cell Fractionation

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13 Terms

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Isotonic
________- this means that the solution contains the correct levels of dissolved salts and sugars.
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ultracentrifuge spins
As the ________, centripetal force pushes the organelles with the most mass to the bottom of the container.
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what is the function of a buffer?
regulates the pH of the solution.
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What is cell fractionation?
the process used to separate cellular components while preserving their individual functions.
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Why is the solution cold?
to reduce enzyme activity
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What is "isotonic"?
the solution contains the correct levels of dissolved salts and sugars
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Buffered
organelles may die or function inefficiently in certain pH values
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Why is the solution isotonic?
prevents water moving in or out of cells by osmosis, preventing organelles from shrivelling up or bursting
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Why is the solution buffered?
some organelles may die or stop functioning at certain pH values
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What force does the ultracentrifuge use?
Centripetal force
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How does the ultracentrifuge work?
Organelles with the greatest mass are pushed the furthest away from the centre, to the bottom of the test tube. Organelles are separated by mass.
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Sediment
Concentrated mass of the heaviest part of the sample, sinks to the bottom
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Supernatant
the clear liquid that lies above a sediment, containing all the other organelles