Cell Fractionation

Isotonic

________- this means that the solution contains the correct levels of dissolved salts and sugars.

ultracentrifuge spins

As the ________, centripetal force pushes the organelles with the most mass to the bottom of the container.

what is the function of a buffer?

regulates the pH of the solution.

What is cell fractionation?

the process used to separate cellular components while preserving their individual functions.

Why is the solution cold?

to reduce enzyme activity

What is "isotonic"?

the solution contains the correct levels of dissolved salts and sugars

Buffered

organelles may die or function inefficiently in certain pH values

Why is the solution isotonic?

prevents water moving in or out of cells by osmosis, preventing organelles from shrivelling up or bursting

Why is the solution buffered?

some organelles may die or stop functioning at certain pH values

What force does the ultracentrifuge use?

Centripetal force

How does the ultracentrifuge work?

Organelles with the greatest mass are pushed the furthest away from the centre, to the bottom of the test tube. Organelles are separated by mass.

Sediment

Concentrated mass of the heaviest part of the sample, sinks to the bottom

Supernatant

the clear liquid that lies above a sediment, containing all the other organelles