Limiting Factors - Temperature and pH

What is the limiting factor for enzyme activity?

Any factor that can slow down or stop the rate of reaction when the level is not optimal

How does temperature affect enzyme activity

It increases rate of reactions as temperature increases

What happens when temperature is too high

Enzyme becomes denatured and causes a sharp decrease in activity

What is the optimal temperature for enzyme activity

The temperature where the enzyme functions most efficiently, around 37 degrees in humans

How does pH affect enzyme activity

It alters the enzyme’s structure and the charge properties of the substrate and enzyme

What happens at extreme pH levels

Enzymes denature and lose their specific shape and functionality

How can enzyme activity be measured

Monitoring the rate of substrate conversion to product