Key Concepts in Chromatography and Atomic Absorption Spectroscopy

These flashcards cover essential terms and concepts related to chromatography and atomic absorption spectroscopy, aiding in exam preparation.

A source of radiation used in atomic absorption spectroscopy.

Hollow Cathode Lamp

The two phases involved in chromatography.

Mobile and Stationary Phases

The Russian botanist who first discovered and used chromatography.

Michael Tsvet

A technique for separation based on differences in solubility.

Partition Chromatography

The property upon which separations are made in partition chromatography.

Solubility

The most commonly used carrier gases in gas chromatography.

Helium and Nitrogen

The basis for separation in gas chromatography.

Relative Volatilities

The phase where less volatile compounds spend more time in gas chromatography.

Stationary Phase

The number of resistors in a thermal conductivity detector.

4

The value equal to 1.5 required for ideal baseline resolution in gas chromatography.

Rs

A term used to calculate the efficiency of a column in gas chromatography.

Theoretical Plates

Refers to liquid chromatography using a polar stationary phase with a non-polar mobile phase.

Normal Phase Separation

An instrument that cannot determine certain metallic species, such as copper.

Flame Photometer

The correct sequence of events that occurs in the flame of a flame photometer.

Vaporization > Desolvation > Atomization > Excitation

A technique where the amount of light absorbed increases with the concentration of the metal species.

Atomic Absorption Spectroscopy

A method that can interface with a mass spectrometer and involves high energy radio frequency.

Plasma Emission Spectroscopy

A sample preparation method that is faster and safer than traditional methods.

Microwave Digestion

The correct sequence of components in an atomic absorption spectrometer.

Source Lamp > Flame > Monochromator > Detector

A measure that indicates the solubility of a solute in two different layers.

Distribution Coefficient

A type of chromatography where separations are based upon molecular weight.

Size Exclusion Chromatography

A method that makes separations based on differences in polarity.

Adsorption Chromatography

These have downsides such as limited sample size in gas chromatography.

Capillary Columns

Universal Detector

A type of detector in gas chromatography that can detect all types of compounds.

Retention Time

It decreases as column temperature increases in gas chromatography.

Theoretical Plates and Height Equivalent

They have an inverse relationship.

High Performance Liquid Chromatograph Components

The correct sequence is Injector > Pump > Column > Detector.

Detection Methods in High Performance Liquid Chromatography

Electron capture is not typically used in high performance liquid chromatography.